Rabbit Polyclonal HSD17B4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B4 aa 350-650.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HSD17B4 aa 350-650. The exact immunogen used to generate this antibody is proprietary information. Database link P51659
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from straight-chain, 2-methyl-branched-chain fatty acids bile acid intermediates. With EHHADH, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:10671535).
Alternative names
Peroxisomal multifunctional enzyme type 2, MFE-2, 17-beta-hydroxysteroid dehydrogenase 4, D-bifunctional protein, Multifunctional protein 2, Short chain dehydrogenase/reductase family 8C member 1, 17-beta-HSD 4, DBP, MFP-2, SDR8C1, HSD17B4, EDH17B4
Recommended products
Rabbit Polyclonal HSD17B4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B4 aa 350-650.
Alternative names
Peroxisomal multifunctional enzyme type 2, MFE-2, 17-beta-hydroxysteroid dehydrogenase 4, D-bifunctional protein, Multifunctional protein 2, Short chain dehydrogenase/reductase family 8C member 1, 17-beta-HSD 4, DBP, MFP-2, SDR8C1, HSD17B4, EDH17B4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HSD17B4 aa 350-650. The exact immunogen used to generate this antibody is proprietary information. Database link P51659
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-HSD17B4 antibody (ab97975)
7.5% SDS PAGE
All lanes: Western blot - Anti-HSD17B4 antibody (ab97975) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)
Immunohistochemical analysis of HSD17B4 in paraffin-embedded DLD-1 (human) xenograft, using ab97975 at 1/500 dilution.
Western blot - Anti-HSD17B4 antibody (ab97975)
All lanes: Western blot - Anti-HSD17B4 antibody (ab97975) at 1/3000 dilution
All lanes: Rat heart lysate at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)
ab97975 staining HSD17B4 in rat heart tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)
ab97975 staining HSD17B4 in rat testis tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HSD17B4 antibody (ab97975)
ab97975 staining HSD17B4 in methanol- fixed Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence) at a 1:500 dilution.
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