Rabbit Polyclonal HSD17B4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B4 aa 350-650.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HSD17B4 aa 350-650. The exact immunogen used to generate this antibody is proprietary information. Database link P51659
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Bifunctional enzyme acting on the peroxisomal fatty acid beta-oxidation pathway. Catalyzes two of the four reactions in fatty acid degradation: hydration of 2-enoyl-CoA (trans-2-enoyl-CoA) to produce (3R)-3-hydroxyacyl-CoA, and dehydrogenation of (3R)-3-hydroxyacyl-CoA to produce 3-ketoacyl-CoA (3-oxoacyl-CoA), which is further metabolized by SCPx. Can use straight-chain and branched-chain fatty acids, as well as bile acid intermediates as substrates.
Alternative names
EDH17B4, SDR8C1, HSD17B4, Peroxisomal multifunctional enzyme type 2, MFE-2, 17-beta-hydroxysteroid dehydrogenase 4, D-bifunctional protein, Multifunctional protein 2, Short chain dehydrogenase/reductase family 8C member 1, 17-beta-HSD 4, DBP, MFP-2
Recommended products
Rabbit Polyclonal HSD17B4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSD17B4 aa 350-650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HSD17B4 aa 350-650. The exact immunogen used to generate this antibody is proprietary information. Database link P51659
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-HSD17B4 antibody (ab97975)
7.5% SDS PAGE
All lanes: Western blot - Anti-HSD17B4 antibody (ab97975) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)
Immunohistochemical analysis of HSD17B4 in paraffin-embedded DLD-1 (human) xenograft, using ab97975 at 1/500 dilution.
Western blot - Anti-HSD17B4 antibody (ab97975)
All lanes: Western blot - Anti-HSD17B4 antibody (ab97975) at 1/3000 dilution
All lanes: Rat heart lysate at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)
ab97975 staining HSD17B4 in rat heart tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B4 antibody (ab97975)
ab97975 staining HSD17B4 in rat testis tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections) at a 1:500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-HSD17B4 antibody (ab97975)
ab97975 staining HSD17B4 in methanol- fixed Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence) at a 1:500 dilution.
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