Anti-HSD3B1 antibody [3C11-D4]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B1 antibody [3C11-D4] (AB55268)

HSD3B1 antibody (ab55268) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.

This image was generated using the ascites version of the product.

• Flow Cyt

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Flow Cytometry - Anti-HSD3B1 antibody [3C11-D4] (AB55268)

Overlay histogram showing JEG3 cells stained with ab55268 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55268, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-HSD3B1 antibody [3C11-D4] (AB55268)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-HSD3B1 antibody [3C11-D4] (ab55268) at 1 µg/mL

All lanes:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa,73 kDa

true

Exposure time: 4min