AB167417

Anti-HSD3B1 antibody [EPR9687]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal HSD3B1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

3BH, HSDB3A, HSD3B1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, Delta-5-3-ketosteroid isomerase, Dihydrotestosterone oxidoreductase, Steroid Delta-isomerase, Trophoblast antigen FDO161G, 3-beta-HSD I

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HSD3B1 antibody [EPR9687] (AB167417)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSD3B1 antibody [EPR9687] (AB167417)

Immunofluorescent analysis of Caco-2 cells labeling HSD3B1 with ab167417 at 1/250 dilution.

Unknown

Western blot - Anti-HSD3B1 antibody [EPR9687] (AB167417)

All lanes:

Western blot - Anti-HSD3B1 antibody [EPR9687] (ab167417) at 1/1000 dilution

All lanes:

Human placenta cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9687

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 83% homology and 6 continuous identical amino acids with HSD3B2(SwissProt: P26439). Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed : 1401999, PubMed : 2139411). Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).

See full target information HSD3B1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Zoological research 46:1108-1120 PubMed41017397

2025

CircSHOC2 regulates steroid hormone synthesis in ovarian granulosa cells through the mir-130b-5p/ASH1L pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Tong Zhang,Teng Zhang,Xiang-Rong Song,Madaniyati Mie-Lie,Yi-Hao Li,Lei Gao,Gong-She Yang,Gui-Yan Chu

Reproductive biomedicine online 50:104296 PubMed39626468

2024

Characteristics of polycystic ovary syndrome rat models induced by letrozole, testosterone propionate and high-fat diets.

Applications

Unspecified application

Species

Unspecified reactive species

Runan Hu,Yanjing Huang,Zhuo Liu,Haoxu Dong,Wenwen Ma,Kunkun Song,Xiaohu Xu,Xiao Wu,Yuli Geng,Fan Li,Mingmin Zhang,Yufan Song

International journal of molecular sciences 22: PubMed33445576

2021

Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Fraichard,Fidéline Bonnet-Serrano,Christelle Laguillier-Morizot,Marylise Hebert-Schuster,René Lai-Kuen,Jeanne Sibiude,Thierry Fournier,Marie Cohen,Jean Guibourdenche

Nanomaterials (Basel, Switzerland) 10: PubMed32635405

2020

Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Margaux Nedder,Sonja Boland,Stéphanie Devineau,Amal Zerrad-Saadi,Jasmina Rogozarski,René Lai-Kuen,Ibtissem Baya,Jean Guibourdenche,Francoise Vibert,Audrey Chissey,Sophie Gil,Xavier Coumoul,Thierry Fournier,Ioana Ferecatu

Journal of applied toxicology : JAT 40:947-964 PubMed32072669

2020

Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia Villela E Silva,Cibele Dos Santos Borges,Josiane de Lima Rosa,Tainá Louise Pacheco,Thamiris Moreira Figueiredo,Gabriel Adan Araújo Leite,Marina Trevizan Guerra,Janete Aparecida Anselmo-Franci,Gary Robert Klinefelter,Wilma De Grava Kempinas

Molecular and cellular endocrinology 499:110586 PubMed31539598

2019

Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation.

Applications

Unspecified application

Species

Unspecified reactive species

C Fraichard,F Bonnet,A Garnier,M Hébert-Schuster,A Bouzerara,P Gerbaud,I Ferecatu,T Fournier,I Hernandez,S Trabado,J Guibourdenche

Molecular and cellular biology 39: PubMed30988160

2019

Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.

Applications

Unspecified application

Species

Unspecified reactive species

David McClellan,Mattie J Casey,Diana Bareyan,Helena Lucente,Christopher Ours,Matthew Velinder,Jason Singer,Mehraju Din Lone,Wenxiang Sun,Yunuen Coria,Clinton C Mason,Michael E Engel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSD3B1 antibody [EPR9687]

Immunocytochemistry/ Immunofluorescence - Anti-HSD3B1 antibody [EPR9687] (AB167417)

Western blot - Anti-HSD3B1 antibody [EPR9687] (AB167417)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (7)

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