Rabbit Polyclonal HSD3B2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSD3B2 aa 100 to C-terminus.
Images
Publications
- Clinical chemistry 69:149-1592022In Situ Metabolomics of Cortisol-Producing Adenomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasanori Murakami,Na Sun,Fengxia Li,Annette Feuchtinger,Celso Gomez-Sanchez,Martin Fassnacht,Martin Reincke,Irina Bancos,Axel Walch,Matthias Kroiss,Felix BeuschleinPubMed 36544353
- Frontiers in cell and developmental biology 7:2862019Bone Morphogenetic Protein 15 Knockdown Inhibits Porcine Ovarian Follicular Development and Ovulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Qin et. al.PubMed 31803742
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HSD3B2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P26439
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Alternative names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD adrenal and gonadal type, 3-beta-HSD II, HSD3B2, HSDB3B
Recommended products
Rabbit Polyclonal HSD3B2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSD3B2 aa 100 to C-terminus.
Alternative names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD adrenal and gonadal type, 3-beta-HSD II, HSD3B2, HSDB3B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HSD3B2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P26439
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
HSD3B2 and HSD3B1 proteins are quite similar in sequence so this product may react with both proteins.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (ab154385)
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling HSD3B2 with ab154385 at 1/100 dilution.
Western blot - Anti-HSD3B2 antibody (ab154385)
10% SDS PAGE
All lanes: Western blot - Anti-HSD3B2 antibody (ab154385) at 1/1000 dilution
Lane 1: Raji whole cell lysate at 30 µg
Lane 2: HeLaS3 whole cell lysate at 30 µg
Predicted band size: 42 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HSD3B2 antibody (ab154385)
Immunofluorescent analysis of methanol-fixed HeLa cells, labeling HSD3B2 with ab154385 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
This image is courtesy of a customer review submitted by Hilde NelisImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (ab154385)
ab154385 staining HSD3B2 in horse cumulus cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% bovine serum for 1 hour at 18°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 2% BSA) for 18 hours at 18°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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