Anti-HSD3B2 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSD3B2 antibody (AB154385)

Immunofluorescent analysis of methanol-fixed HeLa cells, labeling HSD3B2 with ab154385 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (AB154385)

Immunohistochemical analysis of paraffin embedded mouse adrenal gland tissue stained for HSD3B2 protein at cytoplasm with ab154385 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-HSD3B2 antibody (AB154385)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-HSD3B2 antibody (ab154385) at 1/1000 dilution

Lane 1:

Mouse adrenal gland tissue lysates at 50 µg

Lane 2:

Mouse muscle tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 42 kDa

false

• IHC-P

AbReview42024****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (AB154385)

ab154385 staining HSD3B2 in horse cumulus cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% bovine serum for 1 hour at 18°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 2% BSA) for 18 hours at 18°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Hilde Nelis