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Rabbit Recombinant Monoclonal HSDL2 antibody. Suitable for IP, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-HSDL2 antibody [EPR13875(2)] (AB187154), expandable thumbnail
  • Western blot - Anti-HSDL2 antibody [EPR13875(2)] (AB187154), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30 - 1/40
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Has apparently no steroid dehydrogenase activity (PubMed:19703561). Controls bile acid (BA) and lipid metabolism in response to nutritional cues (PubMed:38820148).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal HSDL2 antibody. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13875(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunoprecipitation - Anti-HSDL2 antibody [EPR13875(2)] (ab187154), expandable thumbnail

    Immunoprecipitation - Anti-HSDL2 antibody [EPR13875(2)] (ab187154)

    Immunoprecipitation of HSDL2 from HeLa cells using ab187154 at 1/50 dilution (Lane 1) or PBS alone (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used at 1/500 for detection.

    All lanes: Immunoprecipitation - Anti-HSDL2 antibody [EPR13875(2)] (ab187154)

    Predicted band size: 45 kDa

  • Western blot - Anti-HSDL2 antibody [EPR13875(2)] (ab187154), expandable thumbnail

    Western blot - Anti-HSDL2 antibody [EPR13875(2)] (ab187154)

    All lanes: Western blot - Anti-HSDL2 antibody [EPR13875(2)] (ab187154) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: 293 cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 45 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com