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AB181174

Anti-HSDL2 antibody [EPR13876(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal HSDL2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C9orf99, SDR13C1, HSDL2, Hydroxysteroid dehydrogenase-like protein 2, Short chain dehydrogenase/reductase family 13C member 1

4 Images
Western blot - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)
  • WB

Supplier Data

Western blot - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)

All lanes:

Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)

Immunofluorescent staining of -20°C Acetone fixed HeLa cells labeling HSDL2 using ab181174 at a 1/100 dilution and a Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSDL2 using ab181174 at a 1/100 dilution using prediluted HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)
  • WB

Supplier Data

Western blot - Anti-HSDL2 antibody [EPR13876(B)] (AB181174)

All lanes:

Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/10000 dilution

All lanes:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13876(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has apparently no steroid dehydrogenase activity (PubMed : 19703561). Controls bile acid (BA) and lipid metabolism in response to nutritional cues (PubMed : 38820148).
See full target information HSDL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical journal of experimental and clinical research 24:3997-4008 PubMed29894468

2018

Role of Hydroxysteroid Dehydrogenase-Like 2 (HSDL2) in Human Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Sun,Yilin Zhang,Juanjuan Su,Tiechen Li,Yuxin Jiang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB187154

Anti-HSDL2 antibody [EPR13875(2)]

primary-antibodies

hsdl2-antibody-epr138752-ab187154

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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