Anti-hSET1/SET1 antibody [EPR26821-25] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [EPR26821-25] - BSA and Azide free (AB307159)

This data was developed using ab307158, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling hSET1/SET1 with ab307158 at 1/500 (0.996 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (ab209101). Nuclear staining in mouse testis. The section was incubated with ab307158 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-hSET1/SET1 antibody [EPR26821-25] - BSA and Azide free (AB307159)

This data was developed using ab307158, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling hSET1/SET1 with ab307158 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-hSET1/SET1 antibody [EPR26821-25] - BSA and Azide free (AB307159)

This data was developed using ab307158, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 180 seconds

All lanes:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Observed band size: 280 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-hSET1/SET1 antibody [EPR26821-25] - BSA and Azide free (AB307159)

This data was developed using ab307158, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 180 seconds

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts) whole cell lysate at 20 µg

Observed band size: 280 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-hSET1/SET1 antibody [EPR26821-25] - BSA and Azide free (AB307159)

This data was developed using ab307158, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Anti-NUP98 antibody (ab124980) loading control staining at 1/10000 dilution. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 180 seconds

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) nuclear fraction at 20 µg

Lane 2:

NIH/3T3 cytoplasmic fraction

Observed band size: 280 kDa

false

Exposure time: 180s