Rat Monoclonal HSF1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 15 publications.
Images
![Flow Cytometry - Anti-HSF1 antibody [10H8] (AB61382), expandable thumbnail](https://content.abcam.com/products/images/hsf1-antibody-10h8-ab61382--flow-cytometry-img114670.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-HSF1 antibody [10H8] (AB61382)")
![Western blot - Anti-HSF1 antibody [10H8] (AB61382), expandable thumbnail](https://content.abcam.com/products/images/hsf1-antibody-10h8-ab61382--western-blot-img100248.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HSF1 antibody [10H8] (AB61382)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 antibody [10H8] (AB61382), expandable thumbnail](https://content.abcam.com/products/images/hsf1-antibody-10h8-ab61382--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133050.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 antibody [10H8] (AB61382)")
Publications
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- Clinical and experimental pharmacology & physiology 50:698-7072023Geldanamycin ameliorates multiple organ dysfunction and microthrombosis in septic mice by inhibiting the formation of the neutrophil extracellular network by activating heat shock factor 1 HSF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Li,Ruijing Xuan,Weidong Wu,Hailong Zhang,Jie Zhao,Shan ZhangPubMed 37308449
- Cell reports 42:1121572023Minimal upstream open reading frame of Per2 mediates phase fitness of the circadian clock to day/night physiological body temperature rhythm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakahito Miyake,Yuichi Inoue,Xinyan Shao,Takehito Seta,Yuto Aoki,Khanh Tien Nguyen Pham,Yuichi Shichino,Junko Sasaki,Takehiko Sasaki,Masahito Ikawa,Yoshiaki Yamaguchi,Hitoshi Okamura,Shintaro Iwasaki,Masao DoiPubMed 36882059
- Experimental and therapeutic medicine 22:8202021Significant effects of polysaccharide on lipid metabolism in diabetes may be associated with the activation of the FAM3C-HSF1-CAM signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Pan,Jian Lou,Ling WeiPubMed 34131443
- FEBS open bio 10:1135-11482020HSF1 is required for induction of mitochondrial chaperones during the mitochondrial unfolded protein response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpit Katiyar et. al.PubMed 32302062
- Life sciences 241:1171202019HMGB1 was negatively regulated by HSF1 and mediated the TLR4/MyD88/NF-κB signal pathway in asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiqun Shang,Li Wang,Xiaolan Shi,Ning Wang,Long Zhao,Jing Wang,Cuicui LiuPubMed 31825792
- Medical science monitor : international medical jo 24:2700-27102018Downregulation of MiR-199b-5p Inducing Differentiation of Bone-Marrow Mesenchymal Stem Cells (BMSCs) Toward Cardiomyocyte-Like Cells via HSF1/HSP70 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangjie Dai et. al.PubMed 29715263
- Nature methods 13:938-9442016Directing cellular information flow via CRISPR signal conductors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuchen Liu,Yonghao Zhan,Zhicong Chen,Anbang He,Jianfa Li,Hanwei Wu,Li Liu,Chengle Zhuang,Junhao Lin,Xiaoqiang Guo,Qiaoxia Zhang,Weiren Huang,Zhiming CaiPubMed 27595406
- PloS one 11:e01616152016Loading-Induced Heat-Shock Response in Bovine Intervertebral Disc Organ Culture.
- International journal of molecular medicine 37:56-622016The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress.Applications:WBReactive species:Unspecified reactive speciesShu Tang,Hongbo Chen,Yanfen Cheng,Mohammad Abdel Nasir,Nicole Kemper,Endong BaoPubMed 26719858
- Nature communications 6:65802015Mitochondrial SSBP1 protects cells from proteotoxic stresses by potentiating stress-induced HSF1 transcriptional activity.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Hamster | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage (PubMed:11447121, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:18451878, PubMed:1871105, PubMed:1986252, PubMed:25963659, PubMed:26754925, PubMed:7623826, PubMed:7760831, PubMed:8940068, PubMed:8946918, PubMed:9121459, PubMed:9341107, PubMed:9499401, PubMed:9535852, PubMed:9727490). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:11583998, PubMed:16278218, PubMed:9727490). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:1871105, PubMed:1986252, PubMed:25963659, PubMed:26754925, PubMed:7623826, PubMed:7935471, PubMed:8455624, PubMed:8940068, PubMed:9499401, PubMed:9727490). Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival (PubMed:18451878). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Activates transcription of transcription factor FOXR1 which in turn activates transcription of the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2 (PubMed:34723967). Also serves several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Also plays a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925). (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300.
Alternative names
HSTF1, HSF1, Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1
Recommended products
Rat Monoclonal HSF1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 10H8
- Purification technique
- Affinity purification Protein G
- Specificity
Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Flow Cytometry - Anti-HSF1 antibody [10H8] (ab61382), expandable thumbnail](https://content.abcam.com/products/images/hsf1-antibody-10h8-ab61382--flow-cytometry-img114670.jpg "Flow Cytometry - Anti-HSF1 antibody [10H8] (ab61382)")
Flow Cytometry - Anti-HSF1 antibody [10H8] (ab61382)
Overlay histogram showing HeLa cells stained with ab61382 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61382, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (Rat IgG1, kappa monoclonal [RTK2071] - Isotype control ab18412, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Western blot - Anti-HSF1 antibody [10H8] (ab61382), expandable thumbnail](https://content.abcam.com/products/images/hsf1-antibody-10h8-ab61382--western-blot-img100248.jpg "Western blot - Anti-HSF1 antibody [10H8] (ab61382)")
Western blot - Anti-HSF1 antibody [10H8] (ab61382)
Lanes 1 - 4: Merged signal (red and green). Green - ab61382 observed at 57 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.ab61382 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in Hsf1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsf1 knockout samples were subjected to SDS-PAGE. ab61382 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HSF1 antibody [10H8] (ab61382) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Hsf1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: K562 whole cell lysate at 20 µg
Predicted band size: 57 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 antibody [10H8] (ab61382), expandable thumbnail](https://content.abcam.com/products/images/hsf1-antibody-10h8-ab61382--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133050.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 antibody [10H8] (ab61382)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 antibody [10H8] (ab61382)
IHC image of HSF1 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61382, 5μg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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