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AB284770

Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal HSF1 phospho S303 + S307 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

HSTF1, HSF1, Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)

This data was developed using ab81281 the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling HSF1 with ab81281 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human breast carcinoma without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

Immunocytochemistry - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)
  • ICC

Supplier Data

Immunocytochemistry - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)

This data was developed using ab81281 the same antibody clone in a different buffer.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HSF1 with ab81281 at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of HeLa cells. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)
  • WB

Supplier Data

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)

This data was developed using ab81281 the same antibody clone in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (<a href='/en-us/products/primary-antibodies/hsf1-phospho-s303-s307-antibody-ep1711y-ab81281'>ab81281</a>) at 1/50000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

K-562 whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 76 kDa

false

Exposure time: 100s

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)
  • WB

Supplier Data

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)

This data was developed using ab81281 the same antibody clone in a different buffer. Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (<a href='/en-us/products/primary-antibodies/hsf1-phospho-s303-s307-antibody-ep1711y-ab81281'>ab81281</a>) at 1/50000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 76 kDa

false

Exposure time: 180s

SDS-PAGE - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free (AB284770)

This data was developed using ab81281 the same antibody clone in a different buffer. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa cells labelling HSF1 with ab81281 at 1/90 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.

  • Unconjugated

    Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1711Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

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Anti-Hsp 90 alpha antibody [EPR27270-11]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp 90 alpha antibody [EPR27270-11] (AB303516)

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Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
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Primary Antibodies

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

ab284770 is the carrier-free version of ab81281.

This product has switched from a hybridoma to recombinant production method on 30th May 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage (PubMed : 11447121, PubMed : 12659875, PubMed : 12917326, PubMed : 15016915, PubMed : 18451878, PubMed : 1871105, PubMed : 1986252, PubMed : 25963659, PubMed : 26754925, PubMed : 7623826, PubMed : 7760831, PubMed : 8940068, PubMed : 8946918, PubMed : 9121459, PubMed : 9341107, PubMed : 9499401, PubMed : 9535852, PubMed : 9727490). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed : 11583998, PubMed : 16278218, PubMed : 9727490). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed : 10359787, PubMed : 11583998, PubMed : 12659875, PubMed : 16278218, PubMed : 1871105, PubMed : 1986252, PubMed : 25963659, PubMed : 26754925, PubMed : 7623826, PubMed : 7935471, PubMed : 8455624, PubMed : 8940068, PubMed : 9499401, PubMed : 9727490). Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival (PubMed : 18451878). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed : 11583998, PubMed : 16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed : 1986252, PubMed : 26727489). Binds to chromatin at heat shock gene promoters (PubMed : 25963659). Activates transcription of transcription factor FOXR1 which in turn activates transcription of the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2 (PubMed : 34723967). Also serves several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed : 9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed : 14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed : 17897941). Plays a role in the regulation of mitotic progression (PubMed : 18794143). Also plays a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed : 26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed : 26754925).. (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300.
See full target information HSF1 pS303 + S307
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Product promise

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