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AB327221

Anti-HSF2 Antibody

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Rabbit Polyclonal HSF2 antibody. Suitable for WB, IHC-P, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSF2.

View Alternative Names

HSTF2, HSF2, Heat shock factor protein 2, HSF 2, Heat shock transcription factor 2, HSTF 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF2 Antibody (AB327221)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF2 Antibody (AB327221)

Immunohistochemical staining of human cerebellum using ab327221 at 1 : 50–1 : 200 dilution, showing moderate cytoplasmic positivity in Purkinje cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF2 Antibody (AB327221)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF2 Antibody (AB327221)

Immunohistochemical staining of human testis using ab327221 at 1 : 50–1 : 200 dilution, showing weak to moderate nuclear positivity in cells in seminiferous ducts.

ChIP - Anti-HSF2 Antibody (AB327221)
  • ChIP

Supplier Data

ChIP - Anti-HSF2 Antibody (AB327221)

ChIP-Exo-Seq composite graph for ab327221 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF2 Antibody (AB327221)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF2 Antibody (AB327221)

Immunohistochemical staining of human liver using ab327221 at 1 : 50–1 : 200 dilution, showing no positivity in hepatocytes as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HSF2.

Q03933

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
See full target information HSF2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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