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Rabbit Recombinant Monoclonal Hsp105/HSP110 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 14 publications.


Images

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (AB109624), expandable thumbnail
  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (AB109624), expandable thumbnail
  • Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] (AB109624), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (AB109624), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (AB109624), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Expected
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/50 - 1/500

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10 - 1/100

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (PubMed:24318877). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).

Alternative names

HSP105, HSP110, KIAA0201, HSPH1, HSP110, KIAA0201, HSP105, Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, Heat shock protein family H member 1

Recommended products

Rabbit Recombinant Monoclonal Hsp105/HSP110 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4576

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp105 also known as HSP110 is a member of the heat shock protein family. With a molecular mass of approximately 105 kDa this protein functions as a molecular chaperone. It assists in the proper folding of proteins and prevents aggregation especially under stress conditions. Hsp105 is expressed in various tissues including the brain heart and skeletal muscles indicating its widespread roles in maintaining cellular protein homeostasis.

Biological function summary

Hsp105 plays an essential role in protein folding and stress responses. It forms part of a cellular complex that includes other chaperones like Hsp70 and Hsp40. This interaction is critical in refolding denatured proteins and preventing the accumulation of misfolded proteins. In the cell the chaperone activity of Hsp105 helps in managing protein quality control particularly during heat shock and other stress conditions.

Pathways

This protein integrates within signaling cascades associated with stress responses and apoptosis. It is an important player in the HSF1-mediated heat shock response pathway which regulates the expression of various heat shock proteins. Additionally Hsp105 interacts with proteins like Hsp70 within this pathway coordinating cellular mechanisms that protect against protein-damaging conditions. Another pathway in which Hsp105 is involved might be the ubiquitin-proteasome pathway facilitating the degradation of damaged proteins.

Associated diseases and disorders

Hsp105 has connections to neurodegenerative disorders and cancer. Studies show that its overexpression may link to tumor resistance against chemotherapy by stabilizing proteins involved in cell survival pathways. In neurodegenerative diseases like Alzheimer's Hsp105 binds to tau proteins potentially impacting their normal function and aggregation. It works alongside related proteins such as Hsp70 in these contexts highlighting its role in the pathogenesis and progression of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution

    Lane 1: HeLa whole cell lysate at 20 µg

    Lane 2: HepG2 whole cell lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 83 kDa, 96 kDa

    Observed band size: 105 kDa

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution

    Lane 1: C6 whole cell lysate at 20 µg

    Lane 2: PC-12 whole cell lysate at 20 µg

    Lane 3: NIH/3T3 whole cell lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 96 kDa

    Observed band size: 105 kDa

  • Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    ab109624 (purified) at 1/40 immunoprecipitating Hsp105/HSP110 in NIH/3T3 whole cell lysate.

    Lane 1 (input): NIH/3T3 whole cell lysate (10μg)

    Lane 2 (+): ab109624 + NIH/3T3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109624 in NIH/3T3 whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Predicted band size: 96 kDa

    Observed band size: 105 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    All lanes: Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: Human cerebellum lysate at 10 µg

    Lane 4: MCF-7 cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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