Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/100.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624&44; the same antibody clone in a different buffer formulation.

ab109624 (purified) at 1/40 immunoprecipitating Hsp105/HSP110 in NIH/3T3 whole cell lysate.

Lane 1 (input) : NIH/3T3 whole cell lysate (10μg)

Lane 2 (+) : ab109624 + NIH/3T3 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109624 in NIH/3T3 whole cell lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] (<a href='/en-us/products/primary-antibodies/hsp105-hsp110-antibody-epr4576-ab109624'>ab109624</a>)

Predicted band size: 96 kDa

Observed band size: 105 kDa

false

• WB

Unknown

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (<a href='/en-us/products/primary-antibodies/hsp105-hsp110-antibody-epr4576-ab109624'>ab109624</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human cerebellum lysate at 10 µg

Lane 4:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 96 kDa

false

• WB

Lab

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (<a href='/en-us/products/primary-antibodies/hsp105-hsp110-antibody-epr4576-ab109624'>ab109624</a>) at 1/5000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 83 kDa,96 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] - BSA and Azide free (AB247912)

This data was developed using ab109624, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (<a href='/en-us/products/primary-antibodies/hsp105-hsp110-antibody-epr4576-ab109624'>ab109624</a>) at 1/5000 dilution

Lane 1:

C6 whole cell lysate at 20 µg

Lane 2:

PC-12 whole cell lysate at 20 µg

Lane 3:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 96 kDa

Observed band size: 105 kDa

false