AB108625

Anti-Hsp105/HSP110 antibody [EPR4577]

RabMAb
Recombinant
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(4 Publications )

Rabbit Recombinant Monoclonal Hsp105/HSP110 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

5 Images

Flow Cytometry (Intracellular) - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells, labeling Hsp105/HSP110 with Purified ab108625 at 1/20 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

ab108625, unpurifed, at 1/100 dilution staining Hsp105/HSP110 in HeLa cells by Immunofluorescence.

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hsp105/HSP110 with Purified ab108625 at 1 : 50 dilution (2.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Purified ab108625 at 1 : 20 dilution (0.6μg) immunoprecipitating Hsp105/HSP110 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab108625 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108625 in HeLa whole cell lysate.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 105 kDa

All lanes:

Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625)

Predicted band size: 96 kDa

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Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 96 kDa

Observed band size: 105 kDa

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Carrier free

Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4577

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "For unpurified use at 1/100 - 1/250.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp105 also known as HSP110 is a member of the heat shock protein family. With a molecular mass of approximately 105 kDa this protein functions as a molecular chaperone. It assists in the proper folding of proteins and prevents aggregation especially under stress conditions. Hsp105 is expressed in various tissues including the brain heart and skeletal muscles indicating its widespread roles in maintaining cellular protein homeostasis.

Biological function summary

Hsp105 plays an essential role in protein folding and stress responses. It forms part of a cellular complex that includes other chaperones like Hsp70 and Hsp40. This interaction is critical in refolding denatured proteins and preventing the accumulation of misfolded proteins. In the cell the chaperone activity of Hsp105 helps in managing protein quality control particularly during heat shock and other stress conditions.

Pathways

This protein integrates within signaling cascades associated with stress responses and apoptosis. It is an important player in the HSF1-mediated heat shock response pathway which regulates the expression of various heat shock proteins. Additionally Hsp105 interacts with proteins like Hsp70 within this pathway coordinating cellular mechanisms that protect against protein-damaging conditions. Another pathway in which Hsp105 is involved might be the ubiquitin-proteasome pathway facilitating the degradation of damaged proteins.

Hsp105 has connections to neurodegenerative disorders and cancer. Studies show that its overexpression may link to tumor resistance against chemotherapy by stabilizing proteins involved in cell survival pathways. In neurodegenerative diseases like Alzheimer's Hsp105 binds to tau proteins potentially impacting their normal function and aggregation. It works alongside related proteins such as Hsp70 in these contexts highlighting its role in the pathogenesis and progression of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release (PubMed : 24318877). Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).

See full target information HSPH1

Alternative Names

HSP105, HSP110, KIAA0201, HSPH1, Heat shock protein 105 kDa, Antigen NY-CO-25, Heat shock 110 kDa protein, Heat shock protein family H member 1

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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Cancers 15: PubMed37444524

2023

Externally Applied Electromagnetic Fields and Hyperthermia Irreversibly Damage Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Obrador,Ali Jihad-Jebbar,Rosario Salvador-Palmer,Rafael López-Blanch,María Oriol-Caballo,María Paz Moreno-Murciano,Enrique A Navarro,Rosa Cibrian,José M Estrela

PLoS genetics 17:e1009539 PubMed33914734

2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Gersing,Yong Wang,Martin Grønbæk-Thygesen,Caroline Kampmeyer,Lene Clausen,Martin Willemoës,Claes Andréasson,Amelie Stein,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

Oncoimmunology 6:e1362527 PubMed29209566

2017

Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Shen,Danfeng Guo,Lixia Weng,Shoujie Wang,Zeyu Ma,Yunshan Yang,Pingli Wang,Jianli Wang,Zhijian Cai

Molecular and cellular biology 35:1390-400 PubMed25645927

2015

HSP105 recruits protein phosphatase 2A to dephosphorylate β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy Yu,Michael Kakunda,Victoria Pham,Jennie R Lill,Pan Du,Matthew Wongchenko,Yibing Yan,Ron Firestein,XiaoDong Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp105/HSP110 antibody [EPR4577]

Flow Cytometry (Intracellular) - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] (AB108625)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (4)

Product promise