Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (AB247705)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hsp105/HSP110 with Purified ab108625 at 1/20 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (AB247705)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hsp105/HSP110 with Purified ab108625 at 1 : 50 dilution (2.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

• IP

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Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (AB247705)

Purified ab108625 at 1 : 20 dilution (0.6μg) immunoprecipitating Hsp105/HSP110 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab108625 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108625 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1 : 1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 105 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

All lanes:

Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] (<a href='/en-us/products/primary-antibodies/hsp105-hsp110-antibody-epr4577-ab108625'>ab108625</a>)

Predicted band size: 96 kDa

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• WB

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Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (AB247705)

Blocking/Diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

All lanes:

Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] (<a href='/en-us/products/primary-antibodies/hsp105-hsp110-antibody-epr4577-ab108625'>ab108625</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 96 kDa

Observed band size: 105 kDa

false