AB79784

Anti-Hsp22/HSPB8 antibody

Name: Anti-Hsp22/HSPB8 antibody (ab79784) | Abcam
Brand: Abcam Limited
SKU: AB79784
Rating: 4 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal Hsp22/HSPB8 antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human HSPB8.

View Alternative Names

CRYAC, E2IG1, HSP22, PP1629, HSPB8, Heat shock protein beta-8, HspB8, Alpha-crystallin C chain, E2-induced gene 1 protein, Heat shock protein family B member 8, Protein kinase H11, Small stress protein-like protein HSP22

4 Images

Supplier Data

Immunocytochemistry - Anti-Hsp22/HSPB8 antibody (AB79784)

Immunocytochemical analysis of human HaCaT cells fixed with 100% cold methanol at -20°C for 10 minutes using ab79784 at 1/100 dilution for 12 hours at 4°C, followed by secondary Goat anti-rabbit (green) at 1/50 dilution for 1-2 hours at RT in the dark.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (AB79784)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (AB79784)

ab79784 at 1/100 dilution staining by Hsp22/HSPB8 in mouse spinal cord tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/10 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (AB79784)

Immunohistochemical analysis of Bouin's fixed paraffin-embedded mouse epithelial tissue using ab79784 at 1/100 dilution for 1 hour at RT, followed by secondary Goat anti-rabbit (green) at 1/50 diltuon for 1 hour at RT.

Supplier Data

Western blot - Anti-Hsp22/HSPB8 antibody (AB79784)

All lanes:

Western blot - Anti-Hsp22/HSPB8 antibody (ab79784) at 20 µg

All lanes:

Rat skeletal muscle lysate

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Native Full Length Protein corresponding to Human HSPB8.

Q9UJY1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp22 also known as HSPB8 is a small heat shock protein with a molecular weight of approximately 22 kDa. Expressed in various tissues including heart skeletal muscle and brain Hsp22 functions as a chaperone in cellular conditions. It prevents aggregation and aids in the refolding of misfolded proteins matching its role among the heat shock family proteins known for stress response.

Biological function summary

Hsp22 plays a critical role in maintaining protein homeostasis as part of a larger chaperone complex. It forms hetero-oligomers with other small heat shock proteins like HSPB1 and HSPB6 enhancing its protein-protective functions. These interactions allow Hsp22 to stabilize cytoskeletal elements and partake in autophagic pathways highlighting its importance during cellular stress conditions.

Pathways

Hsp22 integrates into cellular processes of autophagy and proteostasis. It participates in the chaperone-assisted selective autophagy (CASA) pathway. Here it associates with the cochaperone BAG3 facilitating the degradation of damaged proteins. Hsp22 also plays a role in the heat shock response pathway cooperating with other heat shock proteins like HSP70 to protect cells from damage induced by increased temperatures.

Hsp22 has associations with diseases like myopathy and neurodegeneration. In myopathies mutations in Hsp22 can disrupt normal muscular functions leading to muscle weakness and degeneration. In neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) abnormalities in the function or expression of Hsp22 together with its interaction with proteins such as HSPB1 can contribute to the disease's pathogenic mechanisms highlighting its clinical relevance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the chaperone-assisted selective autophagy (CASA), a crucial process for protein quality control, particularly in mechanical strained cells and tissues such as muscle. Displays temperature-dependent chaperone activity.

See full target information HSPB8

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 142:375-393 PubMed33974137

2021

Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions.

Applications

Unspecified application

Species

Unspecified reactive species

Mridul Johari,Jaakko Sarparanta,Anna Vihola,Per Harald Jonson,Marco Savarese,Manu Jokela,Annalaura Torella,Giulio Piluso,Edith Said,Norbert Vella,Marija Cauchi,Armelle Magot,Francesca Magri,Eleonora Mauri,Cornelia Kornblum,Jens Reimann,Tanya Stojkovic,Norma B Romero,Helena Luque,Sanna Huovinen,Päivi Lahermo,Kati Donner,Giacomo Pietro Comi,Vincenzo Nigro,Peter Hackman,Bjarne Udd

The American journal of pathology 190:2464-2477 PubMed33222991

2020

Heat Shock Protein 27 Enhances SUMOylation of Heat Shock Protein B8 to Accelerate the Progression of Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Wang,Xinyan Zhang,Haiwei Wang,Yang Wang,Peng Chen,Longgang Wang

Scientific reports 9:9715 PubMed31273254

2019

The Homeobox gene, HOXB13, Regulates a Mitotic Protein-Kinase Interaction Network in Metastatic Prostate Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Jiqiang Yao,Yunyun Chen,Duy T Nguyen,Zachary J Thompson,Alexey M Eroshkin,Niveditha Nerlakanti,Ami K Patel,Neha Agarwal,Jamie K Teer,Jasreman Dhillon,Domenico Coppola,Jingsong Zhang,Ranjan Perera,Youngchul Kim,Kiran Mahajan

Acta neuropathologica 135:131-148 PubMed28780615

2017

A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8.

Applications

IHC

Species

Unspecified reactive species

Delphine Bouhy,Manisha Juneja,Istvan Katona,Anne Holmgren,Bob Asselbergh,Vicky De Winter,Tino Hochepied,Steven Goossens,Jody J Haigh,Claude Libert,Chantal Ceuterick-de Groote,Joy Irobi,Joachim Weis,Vincent Timmerman

Neurology 86:391-8 PubMed26718575

2016

Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Roula Ghaoui,Johanna Palmio,Janice Brewer,Monkol Lek,Merrilee Needham,Anni Evilä,Peter Hackman,Per-Harald Jonson,Sini Penttilä,Anna Vihola,Sanna Huovinen,Mikaela Lindfors,Ryan L Davis,Leigh Waddell,Simran Kaur,Con Yiannikas,Kathryn North,Nigel Clarke,Daniel G MacArthur,Carolyn M Sue,Bjarne Udd

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp22/HSPB8 antibody

Immunocytochemistry - Anti-Hsp22/HSPB8 antibody (AB79784)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (AB79784)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (AB79784)

Western blot - Anti-Hsp22/HSPB8 antibody (AB79784)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise