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AB248999

Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Hsp22/HSPB8 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

CRYAC, E2IG1, HSP22, PP1629, HSPB8, Heat shock protein beta-8, HspB8, Alpha-crystallin C chain, E2-induced gene 1 protein, Heat shock protein family B member 8, Protein kinase H11, Small stress protein-like protein HSP22

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A673 cells labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151552 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Gastric carcinoma tissue using ab151552 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151552 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151552 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue using ab151552 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • WB

Unknown

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using ab151552, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (<a href='/en-us/products/primary-antibodies/hsp22-hspb8-antibody-epr9714-ab151552'>ab151552</a>) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

Fetal muscle lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 21 kDa

false

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)
  • WB

Lab

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (AB248999)

This data was developed using the same antibody clone in a different buffer formulation (ab151552).

Lanes 1 - 2 : Merged signal (red and green). Green - ab151552 observed at 212 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab151552 was shown to react with Hsp22/HSPB8 in wild-type HeLa cells in Western blot with loss of signal observed in HSPB8 knockout cell line ab265112 (HSPB8 knockout cell lysate ab257468). Wild-type HeLa and HSPB8 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab151552 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (<a href='/en-us/products/primary-antibodies/hsp22-hspb8-antibody-epr9714-ab151552'>ab151552</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HSPB8 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human HSPB8 (Hsp22) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-hspb8-hsp22-knockout-hela-cell-line-ab265112'>ab265112</a>)

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9714

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248999 is the carrier-free version of ab151552.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp22 also known as HSPB8 is a small heat shock protein with a molecular weight of approximately 22 kDa. Expressed in various tissues including heart skeletal muscle and brain Hsp22 functions as a chaperone in cellular conditions. It prevents aggregation and aids in the refolding of misfolded proteins matching its role among the heat shock family proteins known for stress response.
Biological function summary

Hsp22 plays a critical role in maintaining protein homeostasis as part of a larger chaperone complex. It forms hetero-oligomers with other small heat shock proteins like HSPB1 and HSPB6 enhancing its protein-protective functions. These interactions allow Hsp22 to stabilize cytoskeletal elements and partake in autophagic pathways highlighting its importance during cellular stress conditions.

Pathways

Hsp22 integrates into cellular processes of autophagy and proteostasis. It participates in the chaperone-assisted selective autophagy (CASA) pathway. Here it associates with the cochaperone BAG3 facilitating the degradation of damaged proteins. Hsp22 also plays a role in the heat shock response pathway cooperating with other heat shock proteins like HSP70 to protect cells from damage induced by increased temperatures.

Hsp22 has associations with diseases like myopathy and neurodegeneration. In myopathies mutations in Hsp22 can disrupt normal muscular functions leading to muscle weakness and degeneration. In neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) abnormalities in the function or expression of Hsp22 together with its interaction with proteins such as HSPB1 can contribute to the disease's pathogenic mechanisms highlighting its clinical relevance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Displays temperature-dependent chaperone activity.
See full target information HSPB8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang
View all publications
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Product promise

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