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AB202846

Anti-Hsp25 antibody

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(4 Publications)

Rabbit Polyclonal Hsp27 antibody. Suitable for WB and reacts with Rat, Mouse, Recombinant full length protein - Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Hspb1.

View Alternative Names

Hsp25, Hsp27, Hspb1, Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, Heat shock 27 kDa protein, p25, HSP 25, HSP 27

2 Images
Western blot - Anti-Hsp25 antibody (AB202846)
  • WB

Supplier Data

Western blot - Anti-Hsp25 antibody (AB202846)

All lanes:

Western blot - Anti-Hsp25 antibody (ab202846) at 1/1000 dilution

Lane 1:

PC-12 cell lysate (heat shock)

Lane 2:

3T3 cell lysate (heat shock)

Lane 3:

Hsp25 (mouse), (recombinant)

Predicted band size: 23 kDa

false

Western blot - Anti-Hsp25 antibody (AB202846)
  • WB

CiteAb

Western blot - Anti-Hsp25 antibody (AB202846)

Hsp25 western blot using anti-Hsp25 antibody ab202846. Publication image and figure legend from Nikam, A., Patankar, J. V., et al., 2018, Sci Rep, PubMed 30154499.

ab202846 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab202846 please see the product overview.

Western blot analysis of a proteasomal component (PSMD4), p62, Hsp25 and Hsp70, and of autophagy markers LC 3 I and LC 3 II (full size blots are shown in Supplementary Fig. SF8). (a) Western blots of liver homogenate from control mice (pool of n = 3), 10 weeks DDC treated mice (n = 5) and DDC/fenofibrate co-treated mice (n = 5). Densitometric analysis and quantification normalized to β-tubulin for PSMD4 (b), Hsp70 (c), p62 (d) and Hsp25 (e). Student's t-test was performed to evaluate significance, *p < 0.05; **p < 0.01. Western blots of autophagy markers LC3 I and II in livers of mice treated with DDC (left) and DDC + fenofibrate (right) (full size blots are shown in Supplementary Fig. SF9).

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Hspb1.

P14602

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp25 also known as HSPB1 is a small heat shock protein with a molecular mass of around 25 kDa. It is expressed in various tissues including muscle heart and brain where it plays a protective role in response to stress conditions. Hsp25 functions as a molecular chaperone helping proteins maintain their proper folding and preventing aggregation in cells. This protein also assists in protecting cells from apoptosis especially under conditions of heat shock and oxidative stress making it an important factor in cellular homeostasis.
Biological function summary

Small heat shock proteins like Hsp25 contribute to the stabilization of the cytoskeleton and modulation of actin dynamics. Hsp25 forms oligomeric complexes that interact with other chaperones therefore aiding in protein repair and maintenance processes within cells. Its interaction with various proteins helps in upholding cellular integrity during stress underpinning its involvement in the regulation of cellular stress responses and maintaining stability in cellular structures.

Pathways

The role of Hsp25 emerges in stress response and apoptotic pathways. It interacts with proteins such as Akt and p38 MAPK which are integral to these pathways. The presence of Hsp25 can enhance cellular survival by facilitating the activation of Akt which in turn promotes anti-apoptotic signaling. The interaction of Hsp25 with components of the stress response pathways highlights its critical role in managing cellular stress.

Hsp25 has associations with cancer and neurodegenerative diseases. In cancer its ability to inhibit apoptosis can contribute to tumor progression as cancer cells often escape programmed cell death. Hsp25 levels also rise in neurodegenerative conditions such as Alzheimer's disease where it interacts with tau protein suggesting a protective role against protein aggregation. Its involvement in these diseases indicates that Hsp25 plays a dual role both protective in some contexts and potentially problematic in uncontrolled proliferation scenarios.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization (PubMed : 17661394). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (By similarity).
See full target information Hspb1

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:14639 PubMed37670024

2023

Tmem161a regulates bone formation and bone strength through the P38 MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Nagai,Tomohisa Sekimoto,Syuji Kurogi,Tomomi Ohta,Shihoko Miyazaki,Yoichiro Yamaguchi,Takuya Tajima,Etsuo Chosa,Mai Imasaka,Kumiko Yoshinobu,Kimi Araki,Masatake Araki,Narantsog Choijookhuu,Katsuaki Sato,Yoshitaka Hishikawa,Taro Funamoto

Food & function 10:1007-1016 PubMed30706920

2019

Protective effect of biogenic polyphosphate nanoparticles from Synechococcus sp. PCC 7002 on dextran sodium sulphate-induced colitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxin Feng,Mingyong Zeng,Min Huang,Suqin Zhu,Wei Guo,Haohao Wu

Scientific reports 8:12964 PubMed30154499

2018

The PPARα Agonist Fenofibrate Prevents Formation of Protein Aggregates (Mallory-Denk bodies) in a Murine Model of Steatohepatitis-like Hepatotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Aniket Nikam,Jay V Patankar,Meghana Somlapura,Pooja Lahiri,Vinay Sachdev,Dagmar Kratky,Helmut Denk,Kurt Zatloukal,Peter M Abuja

International journal of molecular medicine 41:1887-1898 PubMed29393371

2018

Resistant starch prevents tumorigenesis of dimethylhydrazine-induced colon tumors via regulation of an ER stress-mediated mitochondrial apoptosis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyu Wang,Peng Wang,Zhigang Xiao
View all publications

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