Rabbit Polyclonal Hsp27 antibody. Suitable for WB and reacts with Rat, Mouse, Recombinant full length protein - Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Hspb1.
View Alternative Names
Hsp25, Hsp27, Hspb1, Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, Heat shock 27 kDa protein, p25, HSP 25, HSP 27
- WB
Supplier Data
Western blot - Anti-Hsp25 antibody (AB202846)
All lanes:
Western blot - Anti-Hsp25 antibody (ab202846) at 1/1000 dilution
Lane 1:
PC-12 cell lysate (heat shock)
Lane 2:
3T3 cell lysate (heat shock)
Lane 3:
Hsp25 (mouse), (recombinant)
Predicted band size: 23 kDa
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- WB
CiteAb
Western blot - Anti-Hsp25 antibody (AB202846)
Hsp25 western blot using anti-Hsp25 antibody ab202846. Publication image and figure legend from Nikam, A., Patankar, J. V., et al., 2018, Sci Rep, PubMed 30154499.
ab202846 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab202846 please see the product overview.
Western blot analysis of a proteasomal component (PSMD4), p62, Hsp25 and Hsp70, and of autophagy markers LC 3 I and LC 3 II (full size blots are shown in Supplementary Fig. SF8). (a) Western blots of liver homogenate from control mice (pool of n = 3), 10 weeks DDC treated mice (n = 5) and DDC/fenofibrate co-treated mice (n = 5). Densitometric analysis and quantification normalized to β-tubulin for PSMD4 (b), Hsp70 (c), p62 (d) and Hsp25 (e). Student's t-test was performed to evaluate significance, *p < 0.05; **p < 0.01. Western blots of autophagy markers LC3 I and II in livers of mice treated with DDC (left) and DDC + fenofibrate (right) (full size blots are shown in Supplementary Fig. SF9).
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Reactivity data
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Supplementary information
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Biological function summary
Small heat shock proteins like Hsp25 contribute to the stabilization of the cytoskeleton and modulation of actin dynamics. Hsp25 forms oligomeric complexes that interact with other chaperones therefore aiding in protein repair and maintenance processes within cells. Its interaction with various proteins helps in upholding cellular integrity during stress underpinning its involvement in the regulation of cellular stress responses and maintaining stability in cellular structures.
Pathways
The role of Hsp25 emerges in stress response and apoptotic pathways. It interacts with proteins such as Akt and p38 MAPK which are integral to these pathways. The presence of Hsp25 can enhance cellular survival by facilitating the activation of Akt which in turn promotes anti-apoptotic signaling. The interaction of Hsp25 with components of the stress response pathways highlights its critical role in managing cellular stress.
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Target data
Publications (4)
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Scientific reports 13:14639 PubMed37670024
2023
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Food & function 10:1007-1016 PubMed30706920
2019
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Unspecified reactive species
Scientific reports 8:12964 PubMed30154499
2018
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Unspecified reactive species
International journal of molecular medicine 41:1887-1898 PubMed29393371
2018
Applications
Unspecified application
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Unspecified reactive species
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