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AB79868

Anti-Hsp27 antibody [8A7]

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(10 Publications)

Mouse Monoclonal Hsp27 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human HSPB1.

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

6 Images
Flow Cytometry - Anti-Hsp27 antibody [8A7] (AB79868)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Hsp27 antibody [8A7] (AB79868)

Overlay histogram showing HeLa cells stained with ab79868 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79868, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [8A7] (AB79868)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [8A7] (AB79868)

ab79868 at 1/100,000 dilution staining Hsp27 in human colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [8A7] (AB79868)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [8A7] (AB79868)

ab79868 (1/100) staining Hsp27 in Mouse epidermis tissue sections by IF (Iimmunofluorescence). Secondary Antibody : FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [8A7] (AB79868)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [8A7] (AB79868)

ab79868 staining Hsp27 in Mouse backskin tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [8A7] (AB79868)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [8A7] (AB79868)

Immunohistochemistry analysis of Mouse backskin tissue using Mouse Anti-Hsp27 Monoclonal Antibody, at 1 : 100 dilution for 1 hour at RT. FITC Goat Anti-Mouse (green) was used as a Secondary Antibody at 1 : 50 dilutionfor 1 hour. Localization : Epidermis.

Western blot - Anti-Hsp27 antibody [8A7] (AB79868)
  • WB

Supplier Data

Western blot - Anti-Hsp27 antibody [8A7] (AB79868)

All lanes:

Western blot - Anti-Hsp27 antibody [8A7] (ab79868) at 1/1000 dilution

All lanes:

Rat lung tissue lysate

Predicted band size: 23 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8A7

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human HSPB1. The exact immunogen used to generate this antibody is proprietary information.

P04792

Specificity

ab79868 recognizes Hsp25 and Hsp27, and cross reacts with alpha B crystallin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1076 PubMed40504332

2025

The role of HSPB1 in modulating ferroptosis in pancreatic cancer via the TP53/SLC7A11/GPX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyu Tian,Qi Liu,Jihui Luo,Ning Ning,Jing Qu

International journal of molecular sciences 23: PubMed36232476

2022

Cardiac Alarmins as Residual Risk Markers of Atherosclerosis under Hypolipidemic Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Viorel I Suica,Elena Uyy,Luminita Ivan,Raluca M Boteanu,Aurel Cerveanu-Hogas,Rune Hansen,Felicia Antohe

Scientific reports 11:3465 PubMed33568769

2021

Elucidating the involvement of apoptosis in postmortem proteolysis in porcine muscles from two production cycles using metabolomics approach.

Applications

Unspecified application

Species

Unspecified reactive species

Danyi Ma,Dong Ho Suh,Jiaying Zhang,Yufan Chao,Alan W Duttlinger,Jay S Johnson,Choong Hwan Lee,Yuan H Brad Kim

BMC veterinary research 16:347 PubMed32962700

2020

Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Hu,Tian Ye,Yanzhen Yang,Ben Liu,Wenya Zheng

Journal of animal physiology and animal nutrition 105:1-13 PubMed32744367

2020

Effects of transport stress on pathological injury and main heat shock protein expression in the respiratory system of goats.

Applications

Unspecified application

Species

Unspecified reactive species

Wenya Zheng,Ben Liu,Wei Hu,Yan Cui

PloS one 12:e0176451 PubMed28463974

2017

The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens).

Applications

WB, IHC

Species

Torpedo, Owl

Xue Yang,Yan Cui,Jing Yue,Honghong He,Chuan Yu,Penggang Liu,Jun Liu,Xiandong Ren,Yun Meng

Veterinary ophthalmology 19:262-6 PubMed26302381

2015

Heat-shock protein expression in canine corneal wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Cornelia W M Peterson,Renee T Carter,Ellison Bentley,Christopher J Murphy,Heather L Chandler

Physiological reports 1:e00127 PubMed24400135

2013

Lysine deacetylase inhibition promotes relaxation of arterial tone and C-terminal acetylation of HSPB6 (Hsp20) in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aiqing Chen,Magdalena Karolczak-Bayatti,Michèle Sweeney,Achim Treumann,Kelly Morrissey,Scott M Ulrich,G Nicholas Europe-Finner,Michael J Taggart

The Journal of biological chemistry 276:39911-8 PubMed11546764

2001

Identification and characterization of hic-5/ARA55 as an hsp27 binding protein.

Applications

IP

Species

Unspecified reactive species

Y Jia,R F Ransom,M Shibanuma,C Liu,M J Welsh,W E Smoyer

Biology of reproduction 55:141-51 PubMed8793069

1996

Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

M J Welsh,W Wu,M Parvinen,R R Gilmont
View all publications
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