Mouse Monoclonal Hsp27 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67232.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499)")
![Western blot - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img140560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 antibody [D2] (AB239499)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111021.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124265.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115336.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04792
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.5-5 µg/mL | Notes - |
Species Human | Dilution info 0.5-5 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-30 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Alternative names
Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, HSP 27, SRP27, HSP28, HSP27, HSPB1
Recommended products
Mouse Monoclonal Hsp27 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus.
Alternative names
Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, HSP 27, SRP27, HSP28, HSP27, HSPB1
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04792
- Clone number
D2
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
- Biological function summary
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
- Pathways
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
- Associated diseases and disorders
Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67232.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)
Paraffin-embedded human breast cancer tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img140560.jpg "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
Western blot - Anti-Hsp27 antibody [D2] (ab239499)
All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 2 µg/mL
All lanes: Pig skeletal muscle lysate
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111021.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)
Paraffin-embedded human glioma tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124265.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)
Paraffin-embedded human brain tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115336.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)
Paraffin-embedded human stomach tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img141572.jpg "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
Western blot - Anti-Hsp27 antibody [D2] (ab239499)
All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 5 µg/mL
All lanes: Recombinant human Hsp27 protein
SecondaryAll lanes: HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 23 kDa
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img102373.jpg "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
Western blot - Anti-Hsp27 antibody [D2] (ab239499)
All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 5 µg/mL
Lane 1: Human lung lysate
Lane 2: Pig muscle lysate
Lane 3: Pig heart lysate
SecondaryAll lanes: HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55965.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)
Paraffin-embedded human kidney tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img138010.jpg "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
Western blot - Anti-Hsp27 antibody [D2] (ab239499)
All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 2 µg/mL
All lanes: Pig heart lysate
Predicted band size: 23 kDa
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img141572.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img102373.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55965.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)")
![Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-d2-ab239499--western-blot-img138010.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 antibody [D2] (ab239499)")
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