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Mouse Monoclonal Hsp27 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail
  • Western blot - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (AB239499), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04792

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Pig
Tested
Expected

Tested
Tested

Species

Pig

Dilution info

0.5-5 µg/mL

Notes

-

Species

Human

Dilution info

0.5-5 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

5-30 µg/mL

Notes

-

Expected
Expected

Species

Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Alternative names

Recommended products

Mouse Monoclonal Hsp27 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus.

Alternative names

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human HSPB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04792
Clone number

D2

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Associated diseases and disorders

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

Product promise

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9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

    Paraffin-embedded human breast cancer tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [D2] (ab239499)

    All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 2 µg/mL

    All lanes: Pig skeletal muscle lysate

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

    Paraffin-embedded human glioma tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

    Paraffin-embedded human brain tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

    Paraffin-embedded human stomach tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [D2] (ab239499)

    All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 5 µg/mL

    All lanes: Recombinant human Hsp27 protein

    Secondary

    All lanes: HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 23 kDa

  • Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [D2] (ab239499)

    All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 5 µg/mL

    Lane 1: Human lung lysate

    Lane 2: Pig muscle lysate

    Lane 3: Pig heart lysate

    Secondary

    All lanes: HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [D2] (ab239499)

    Paraffin-embedded human kidney tissue stained for Hsp27 using ab239499 at 30 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Hsp27 antibody [D2] (ab239499), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [D2] (ab239499)

    All lanes: Western blot - Anti-Hsp27 antibody [D2] (ab239499) at 2 µg/mL

    All lanes: Pig heart lysate

    Predicted band size: 23 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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