Rabbit Recombinant Monoclonal Hsp27 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--western-blot-img140988.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352)")
![Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--western-blot-img137721.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444420.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444418.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (AB247352)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Alternative names
HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27
Recommended products
Rabbit Recombinant Monoclonal Hsp27 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1724Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247352 is the carrier-free version of Anti-Hsp27 antibody [EP1724Y] ab62339.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
- Biological function summary
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
- Pathways
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
- Associated diseases and disorders
Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--western-blot-img140988.jpg "Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp27 antibody [EP1724Y] ab62339).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Hsp27 antibody [EP1724Y] ab62339 observed at 27 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Hsp27 antibody [EP1724Y] ab62339 was shown to specifically react with Hsp27 in wild-type HAP1 cells. No band was observed when Hsp27 knockout samples were used. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. Anti-Hsp27 antibody [EP1724Y] ab62339 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352) at 1/500 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Hsp27 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: MCF7 whole cell lysate at 20 µg
Predicted band size: 23 kDa
![Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--western-blot-img137721.jpg "Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp27 antibody [EP1724Y] ab62339).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Hsp27 antibody [EP1724Y] ab62339 observed at 23 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Hsp27 antibody [EP1724Y] ab62339 was shown to react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human HSPB1 (Hsp27) knockout HeLa cell line ab261738 (knockout cell lysate Human HSPB1 (Hsp27) knockout HeLa cell lysate ab256945) was used. Wild-type HeLa and HSPB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Hsp27 antibody [EP1724Y] ab62339 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp27 antibody [EP1724Y] (Anti-Hsp27 antibody [EP1724Y] ab62339) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: HSPB1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human HSPB1 (Hsp27) knockout HeLa cell line (Human HSPB1 (Hsp27) knockout HeLa cell line ab261738)
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444420.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
This data was developed using Anti-Hsp27 antibody [EP1724Y] ab62339, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat skin labeling Hsp27 with Anti-Hsp27 antibody [EP1724Y] ab62339 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Cytoplasmic staining on rat skin.
The section was incubated with Anti-Hsp27 antibody [EP1724Y] ab62339 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
This data was developed using Anti-Hsp27 antibody [EP1724Y] ab62339, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse skin labeling Hsp27 with Anti-Hsp27 antibody [EP1724Y] ab62339 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Cytoplasmic staining on mouse skin.
The section was incubated with Anti-Hsp27 antibody [EP1724Y] ab62339 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444418.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
This data was developed using Anti-Hsp27 antibody [EP1724Y] ab62339, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skin labeling Hsp27 with Anti-Hsp27 antibody [EP1724Y] ab62339 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Cytoplasmic staining on human skin.
The section was incubated with Anti-Hsp27 antibody [EP1724Y] ab62339 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444417.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
This data was developed using Anti-Hsp27 antibody [EP1724Y] ab62339, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer labeling Hsp27 with Anti-Hsp27 antibody [EP1724Y] ab62339 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Cytoplasmic staining on human breast cancer.
The section was incubated with Anti-Hsp27 antibody [EP1724Y] ab62339 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352), expandable thumbnail](https://content.abcam.com/products/images/hsp27-antibody-ep1724y-bsa-and-azide-free-ab247352--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img444417.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
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