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Rabbit Recombinant Monoclonal Hsp27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, African green monkey samples. Cited in 7 publications.


Images

Western blot - Anti-Hsp27 antibody [EPR5477] (AB109376), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (AB109376), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [EPR5477] (AB109376), expandable thumbnail
  • Western blot - Anti-Hsp27 antibody [EPR5477] (AB109376), expandable thumbnail
  • Western blot - Anti-Hsp27 antibody [EPR5477] (AB109376), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Rat
Predicted
Predicted
Predicted
African green monkey
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Species

African green monkey

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

African green monkey

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 -Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

African green monkey

Dilution info

1/10 - 1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 -Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Alternative names

HSP27, HSP28, HSP28, HSP27, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

Recommended products

Rabbit Recombinant Monoclonal Hsp27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, African green monkey samples. Cited in 7 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5477

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Associated diseases and disorders

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376)

    Lanes 1- 2: Merged signal (red and green). Green - ab109376 observed at 23 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab109376 was shown to react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human HSPB1 (Hsp27) knockout HeLa cell line ab261738 (knockout cell lysate Human HSPB1 (Hsp27) knockout HeLa cell lysate ab256945) was used. Wild-type HeLa and Hsp27 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109376 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: HSPB1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

  • Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (ab109376), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (ab109376)

    Overlay histogram showing wild-type HAP1 (green line) and HSPB1 knockout HAP1 cells (red line) stained with ab109376. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab109376, 1μg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) presorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution for 30 min at 22°C. A rabbit IgG isotype control antibody (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, knockout HSPB1 HAP1- grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [EPR5477] (ab109376), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [EPR5477] (ab109376)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Hsp27 with purified ab109376 at 1/500. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109376 observed at 27 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab109376 was shown to specifically react with Hsp27 in wild-type HAP1 cells as signal was lost in Hsp27 knockout HAP1 cells. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. ab109376 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: Hsp27 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: MCF7 whole cell lysate at 20 µg

    Predicted band size: 23 kDa

  • Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376), expandable thumbnail

    Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376)

    All lanes: Western blot - Anti-Hsp27 antibody [EPR5477] (ab109376) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: COS-1 cell lysate at 10 µg

    Lane 3: BxPC-3 cell lysate at 10 µg

    Lane 4: HT-1376 cell lysate at 10 µg

    Predicted band size: 23 kDa

    Observed band size: 27 kDa

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Product protocols

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