AB76313

Anti-Hsp27 (phospho S15) antibody [EP2293Y]

Name: Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) | Abcam
Brand: Abcam Limited
SKU: AB76313
Price: 195 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal Hsp27 phospho S15 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 9 publications.

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (AB76313)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (AB76313)

ab76313 staining Hsp27 (phospho S15) in HeLa (human cervix adenocarcinoma) cells with heat treatment at 44°C. by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab76313 (1/1000) and secondary antibody ab150077 (1/1000) respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

Lab

Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (AB76313)

All lanes:

Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) at 1/2500 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

UV treated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2293Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.13% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).

See full target information HSPB1 phospho S15

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:470 PubMed40593489

2025

PKIB facilitates bladder cancer proliferation and metastasis through mediation of HSP27 phosphorylation by PKA.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Liu,Xiaoyu Yin,Feng Yuan,Shiqing Li,Fei Xiao,Feng Zhou,Xudong Pan,Yatfaat Ho,Shuo Dong,Duan Xu,Yunqing Ma,Zhengding Cao,Zhe Lei,Yi Sun

MedComm 6:e70032 PubMed40013315

2025

Targeting WDPF domain of Hsp27 achieves a broad spectrum of antiviral.

Applications

Unspecified application

Species

Unspecified reactive species

Mandi Wu,Wei Li,Houying Leung,Yiran Wang,Qianya Wan,Peiran Chen,Cien Chen,Yichen Li,Xi Yao,Ming-Liang He

Scientific reports 14:2856 PubMed38310132

2024

Mechanical activation and expression of HSP27 in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Molly Buckley,Maranda Kramer,Bronte Johnson,Gillian Huskin,Joel Berry,Mary Kathryn Sewell-Loftin

Journal of oncology 2022:3552793 PubMed36385956

2022

Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Fukuchi,Kayoko Nanai,Hiroshi Yuita,Chikako Maru,Jun Tsukada,Masato Ishigami,Yoko Nagai,Yoko Nakano,Chigusa Yoshimura,Kozo Yoneda,Masato Amano,Kensuke Nakamura,Yoko Oda,Haruyuki Nishigohri,Shoji Yamamoto,Yusuke Ohnishi-Totoki,Koichiro Inaki,Hironobu Komori,Rika Nakano,Yoshiyuki Kanari,Atsuko Nishida,Yumi Matsui,Satoko Funo,Sayako Takahashi,Toshiaki Ohtsuka,Toshinori Agatsuma

Journal of applied physiology (Bethesda, Md. : 1985) 130:545-561 PubMed33356984

2020

Expression of titin-linked putative mechanosensing proteins in skeletal muscle after power resistance exercise in resistance-trained men.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan G Wette,Nigel P Birch,Mattias Soop,Martina Zügel,Robyn M Murphy,Graham D Lamb,Heather K Smith

Molecules (Basel, Switzerland) 25: PubMed32570757

2020

FAM83G Is a Novel Inducer of Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junichi Okada,Noriaki Sunaga,Eijiro Yamada,Tsugumichi Saito,Atsushi Ozawa,Yasuyo Nakajima,Kazuya Okada,Jeffrey E Pessin,Shuichi Okada,Masanobu Yamada

Journal of cancer research and clinical oncology 146:1125-1137 PubMed32200459

2020

Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma.

Applications

IHC-P

Species

Human

Richard Drexler,Kim C Wagner,Mirco Küchler,Bernd Feyerabend,Moritz Kleine,Karl J Oldhafer

PloS one 11:e0161975 PubMed27603666

2016

Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro P Adam,Anthony M Lowery,Nina Martino,Hiba Alsaffar,Peter A Vincent

The Journal of biological chemistry 288:6743-53 PubMed23335506

2013

LY2228820 dimesylate, a selective inhibitor of p38 mitogen-activated protein kinase, reduces angiogenic endothelial cord formation in vitro and in vivo.

Applications

Species

Unspecified reactive species

Courtney M Tate,Wayne Blosser,Lisa Wyss,Glenn Evans,Qi Xue,Yong Pan,Louis Stancato

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp27 (phospho S15) antibody [EP2293Y]

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (AB76313)

Western blot - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (AB76313)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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