Rabbit Recombinant Monoclonal Hsp27 phospho S78 antibody. Suitable for IHC-P, ICC/IF, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 10 publications.
Images
![Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--western-blot-img165118.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168233.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)")
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--immunocytochemistry-immunofluorescence-img166772.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)")
![Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--dot-blot-img857.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)")
Publications
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- International journal of molecular sciences 24:2023Targeting Heat Shock Protein 27 and Fatty Acid Oxidation Augments Cisplatin Treatment in Cisplatin-Resistant Ovarian Cancer Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Patrick Heiserman,Zenab Minhas,Elahe Nikpayam,Dong-Joo CheonPubMed 37628819
- Nature cell biology 25:467-4802023Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElias Adriaenssens,Bob Asselbergh,Pablo Rivera-Mejías,Sven Bervoets,Leen Vendredy,Vicky De Winter,Katrien Spaas,Riet de Rycke,Gert van Isterdael,Francis Impens,Thomas Langer,Vincent TimmermanPubMed 36690850
- PloS one 16:e02460502021Pseudomonas aeruginosa induces p38MAP kinase-dependent IL-6 and CXCL8 release from bronchial epithelial cells via a Syk kinase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew S Coates et. al.PubMed 33524056
- British journal of pharmacology 177:5046-50622020Spinal heat shock protein 27 participates in PDGFRβ-mediated morphine tolerance through PI3K/Akt and p38 MAPK signalling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Li,Xiaoling Peng,Xiaoqian Jia,Peng Su,Daiqiang Liu,Ye Tu,Qiaoqiao Xu,Feng GaoPubMed 32559815
- Journal of cancer research and clinical oncology 146:1125-11372020Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma.
- Digestive diseases and sciences :2020Heat Shock Protein 27 Regulates the Inflammatory Response of Intestinal Epithelial Cells by the Nuclear Factor-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYajun Zhang et. al.PubMed 32078087
- Molecular medicine reports 12:3787-37942015Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuo Chu Li et. al.PubMed 26044344
- Journal of molecular signaling 6:42011Distinct roles of MK2 and MK5 in cAMP/PKA- and stress/p38MAPK-induced heat shock protein 27 phosphorylation.
- Molecular & cellular proteomics : MCP 10:M110.0035172011A proteomic focus on the alterations occurring at the human atherosclerotic coronary intima.
- Molecular cancer therapeutics 9:1274-852010IFNgamma restores breast cancer sensitivity to fulvestrant by regulating STAT1, IFN regulatory factor 1, NF-kappaB, BCL2 family members, and signaling to caspase-dependent apoptosis.Applications:WBReactive species:HumanYanxia Ning,Rebecca B Riggins,Jennifer E Mulla,Haniee Chung,Alan Zwart,Robert ClarkePubMed 20457620
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | Dot | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Alternative names
Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, HSP 27, SRP27, HSPB1, HSP27, HSP28
Recommended products
Rabbit Recombinant Monoclonal Hsp27 phospho S78 antibody. Suitable for IHC-P, ICC/IF, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 10 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
Y175
- Purification technique
Affinity purification Protein A
- Specificity
ab32501 recognises Hsp27 (phospho S78). The antibody will detect Src phosphorylation on Serine 78.
This antibody does not react with mouse and rat species in the Western blot application.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
- Biological function summary
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
- Pathways
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
- Associated diseases and disorders
Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--western-blot-img165118.jpg "Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)")
Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)
All lanes: Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 minutes whole cell lysate at 15 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 minutes whole cell lysate, and then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
This data was developed using ab32501, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168233.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labelling Hsp27 with purified ab32501 at 1/5000 dilution (0.11 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. Positive staining on human breast cancer without alkaline phosphatase treatment (image A). No staining on human breast cancer with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--immunocytochemistry-immunofluorescence-img166772.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)")
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 25 ug/mL anisomycin for 30 min, then Lambda Protein Phosphatase 31 for 2 hours cells labeling Hsp27 with purified ab32501 at 1/50 dilution (11.3 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) dilution. Goat anti-rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-ab32501--dot-blot-img857.jpg "Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)")
Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)
Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32501 at dilution 1/2000.
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