AB32501

Anti-Hsp27 (phospho S78) antibody [Y175]

BOND RX™ Validated
RabMAb
Recombinant
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(14 Publications)

Rabbit Recombinant Monoclonal Hsp27 phospho S78 antibody. Suitable for IHC-P, ICC/IF, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 14 publications.

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labelling Hsp27 with purified ab32501 at 1/5000 dilution (0.11 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. Positive staining on human breast cancer without alkaline phosphatase treatment (image A). No staining on human breast cancer with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 25 ug/mL anisomycin for 30 min, then Lambda Protein Phosphatase 31 for 2 hours cells labeling Hsp27 with purified ab32501 at 1/50 dilution (11.3 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) dilution. Goat anti-rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Lab

Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

All lanes:

Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 minutes whole cell lysate at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 minutes whole cell lysate, and then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Unknown

Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32501 at dilution 1/2000.

Carrier free

Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y175

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32501 recognises Hsp27 (phospho S78). The antibody will detect Src phosphorylation on Serine 78.

This antibody does not react with mouse and rat species in the Western blot application.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).

See full target information HSPB1 phospho S78

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:470 PubMed40593489

2025

PKIB facilitates bladder cancer proliferation and metastasis through mediation of HSP27 phosphorylation by PKA.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Liu,Xiaoyu Yin,Feng Yuan,Shiqing Li,Fei Xiao,Feng Zhou,Xudong Pan,Yatfaat Ho,Shuo Dong,Duan Xu,Yunqing Ma,Zhengding Cao,Zhe Lei,Yi Sun

Cellular and molecular life sciences : CMLS 82:87 PubMed39985589

2025

Aminophylline targets miR-128-3p/Slc7a11 axis to attenuate neuronal ferroptosis after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Manrui,Yang Xu,Jinyuan Liu,Xiao Zhang,Ruixuan Yuan,Yuwen Sun,Yihan Sun,Qiuyun Yang,Miao Liao,Meili Lv,Xin Hu,Xiameng Chen,Weibo Liang

Frontiers in oncology 14:1433256 PubMed39717750

2024

Neuroblastoma response to RAS-MAPK inhibitors and APR-246 (eprenetapopt) co-treatment is dependent on SLC7A11.

Applications

Unspecified application

Species

Unspecified reactive species

Vid Mlakar,Ina Oehme,Laurence Lesne,Sara Najafi,Marc Ansari,Fabienne Gumy-Pause

International journal of molecular sciences 24: PubMed37628819

2023

Targeting Heat Shock Protein 27 and Fatty Acid Oxidation Augments Cisplatin Treatment in Cisplatin-Resistant Ovarian Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

James Patrick Heiserman,Zenab Minhas,Elahe Nikpayam,Dong-Joo Cheon

Nature cell biology 25:467-480 PubMed36690850

2023

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Adriaenssens,Bob Asselbergh,Pablo Rivera-Mejías,Sven Bervoets,Leen Vendredy,Vicky De Winter,Katrien Spaas,Riet de Rycke,Gert van Isterdael,Francis Impens,Thomas Langer,Vincent Timmerman

PloS one 16:e0246050 PubMed33524056

2021

Pseudomonas aeruginosa induces p38MAP kinase-dependent IL-6 and CXCL8 release from bronchial epithelial cells via a Syk kinase pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew S Coates,Eric W F W Alton,Garth W Rapeport,Jane C Davies,Kazuhiro Ito

British journal of pharmacology 177:5046-5062 PubMed32559815

2020

Spinal heat shock protein 27 participates in PDGFRβ-mediated morphine tolerance through PI3K/Akt and p38 MAPK signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Li,Xiaoling Peng,Xiaoqian Jia,Peng Su,Daiqiang Liu,Ye Tu,Qiaoqiao Xu,Feng Gao

Journal of cancer research and clinical oncology 146:1125-1137 PubMed32200459

2020

Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma.

Applications

IHC-P

Species

Human

Richard Drexler,Kim C Wagner,Mirco Küchler,Bernd Feyerabend,Moritz Kleine,Karl J Oldhafer

Digestive diseases and sciences : PubMed32078087

2020

Heat Shock Protein 27 Regulates the Inflammatory Response of Intestinal Epithelial Cells by the Nuclear Factor-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Zhang,Xiaohui Wang,Shaoxin Wang,Zhihui Yan,Chao Li,Yan Zheng,Lihong Cui

In vivo (Athens, Greece) 32:637-642 PubMed29695571

2018

Heat Shock Protein 27 Expression in EUS-FNA Samples Can Predict Gemcitabine Sensitivity in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Michitaka Kawano,Seiji Kaino,Shogo Amano,Shuhei Shinoda,Shigeyuki Suenaga,Manabu Sen-Yo,Isao Sakaida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp27 (phospho S78) antibody [Y175]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (AB32501)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Supplementary information

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Target data

Publications (14)

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