Rabbit Recombinant Monoclonal Hsp27 phospho S78 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Dot, WB and reacts with Human, Synthetic peptide samples.
Images
![Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--western-blot-img165118.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168233.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262)")
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--immunocytochemistry-immunofluorescence-img166772.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262)")
![Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--dot-blot-img857.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (AB247262)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | Dot | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Alternative names
HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27
Recommended products
Rabbit Recombinant Monoclonal Hsp27 phospho S78 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Dot, WB and reacts with Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- Y175
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognises Hsp27 (phospho S78). It will detect Src phosphorylation on Serine 78.
This antibody does not react with mouse and rat species in the Western blot application.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247262 is the carrier-free version of Anti-Hsp27 (phospho S78) antibody [Y175] ab32501.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
- Biological function summary
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
- Pathways
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
- Associated diseases and disorders
Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--western-blot-img165118.jpg "Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)")
Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
This data was developed using Anti-Hsp27 (phospho S78) antibody [Y175] ab32501, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (Anti-Hsp27 (phospho S78) antibody [Y175] ab32501) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 minutes whole cell lysate at 15 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 minutes whole cell lysate, and then the menbrane treated with Alkaline Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168233.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
This data was developed using Anti-Hsp27 (phospho S78) antibody [Y175] ab32501, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Hsp27 with purified Anti-Hsp27 (phospho S78) antibody [Y175] ab32501 at 1/5000 dilution (0.11 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--immunocytochemistry-immunofluorescence-img166772.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)")
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
This data was developed using Anti-Hsp27 (phospho S78) antibody [Y175] ab32501, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 25 ug/mL anisomycin for 30 min, then Lambda Protein Phosphatase 31 for 2 hours cells labeling Hsp27 with purified Anti-Hsp27 (phospho S78) antibody [Y175] ab32501 at 1/50 dilution (11.3 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) dilution. Goat anti-rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262), expandable thumbnail](https://content.abcam.com/products/images/hsp27-phospho-s78-antibody-y175-bsa-and-azide-free-ab247262--dot-blot-img857.jpg "Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)")
Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] - BSA and Azide free (ab247262)
This data was developed using Anti-Hsp27 (phospho S78) antibody [Y175] ab32501, the same antibody clone in a different buffer formulation.Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using Anti-Hsp27 (phospho S78) antibody [Y175] ab32501 at dilution 1/2000.
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