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Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 6 publications.

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Images

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987), expandable thumbnail
  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987), expandable thumbnail
  • Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987), expandable thumbnail
  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987), expandable thumbnail
  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (AB155987), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytDotWBIHC-P
Human
Not recommended
Tested
Not recommended
Expected
Tested
Not recommended
Mouse
Not recommended
Expected
Not recommended
Expected
Tested
Not recommended
Rat
Not recommended
Expected
Not recommended
Expected
Tested
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/75
Notes

For unpurified, use 1/10.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

For unpurified, use 1/200.

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

For unpurified, use 1/200.

Species
Human
Dilution info
1/1000 - 1/2000
Notes

For unpurified, use 1/200.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7278
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Associated diseases and disorders

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Predicted band size: 23 kDa

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 23 kDa

    Observed band size: 27 kDa

    Exposure time: 1min

  • Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Dot blot analysis of Lane 1: Hsp27 (pS82) phospho peptide and Lane 2: Hsp27 non-phospho peptide, labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 10 seconds.

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution

    Lane 1: Mouse heart at 10 µg

    Lane 2: Rat heart at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution

    All lanes: HeLa cell lysate treated with heat shock (44°C) at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 27 kDa

  • Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Predicted band size: 23 kDa

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution

    Lane 1: Mouse heart at 10 µg

    Lane 2: Rat heart at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution

    All lanes: HeLa cells treated with heat shock (44°C) at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa, 89 kDa

    Observed band size: 27 kDa, 80 kDa

  • Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987), expandable thumbnail

    Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution

    Lane 1: Untreated HeLa cell lysate at 10 µg

    Lane 2: HeLa cell lysate treated with heat shock (44°C) at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 15 kDa, 216 kDa, 23 kDa, 28 kDa, 286 kDa

    Observed band size: 15 kDa, 260 kDa, 48 kDa

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Product protocols

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