Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 6 publications.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ICC/IF | IP | Flow Cyt | Dot | WB | IHC-P | |
---|---|---|---|---|---|---|
Human | Not recommended | Tested | Not recommended | Expected | Tested | Not recommended |
Mouse | Not recommended | Expected | Not recommended | Expected | Tested | Not recommended |
Rat | Not recommended | Expected | Not recommended | Expected | Tested | Not recommended |
Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/75 | Notes For unpurified, use 1/10. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes For unpurified, use 1/200. |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes For unpurified, use 1/200. |
Species Human | Dilution info 1/1000 - 1/2000 | Notes For unpurified, use 1/200. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
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Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27
Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 6 publications.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)
Predicted band size: 23 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 1min
Dot blot analysis of Lane 1: Hsp27 (pS82) phospho peptide and Lane 2: Hsp27 non-phospho peptide, labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 10 seconds.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution
Lane 1: Mouse heart at 10 µg
Lane 2: Rat heart at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution
All lanes: HeLa cell lysate treated with heat shock (44°C) at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)
Predicted band size: 23 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution
Lane 1: Mouse heart at 10 µg
Lane 2: Rat heart at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution
All lanes: HeLa cells treated with heat shock (44°C) at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 23 kDa, 89 kDa
Observed band size: 27 kDa, 80 kDa
All lanes: Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution
Lane 1: Untreated HeLa cell lysate at 10 µg
Lane 2: HeLa cell lysate treated with heat shock (44°C) at 10 µg
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa, 216 kDa, 23 kDa, 28 kDa, 286 kDa
Observed band size: 15 kDa, 260 kDa, 48 kDa
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