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AB249232

Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

9 Images
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • WB

Lab

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Exposure time: 1min

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • WB

Lab

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>) at 1/200 dilution

Lane 1:

Mouse heart at 10 µg

Lane 2:

Rat heart at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • IP

Lab

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>)

Predicted band size: 23 kDa

false

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • IP

Lab

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>)

Predicted band size: 23 kDa

false

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • WB

Lab

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>) at 1/200 dilution

All lanes:

HeLa cells treated with heat shock (44°C) at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa,89 kDa

Observed band size: 27 kDa,80 kDa

false

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • WB

Lab

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>) at 1/1500 dilution

Lane 1:

Mouse heart at 10 µg

Lane 2:

Rat heart at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • WB

Lab

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>) at 1/1500 dilution

All lanes:

HeLa cell lysate treated with heat shock (44°C) at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • WB

Unknown

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (<a href='/en-us/products/primary-antibodies/hsp27-phospho-s82-antibody-epr7278-ab155987'>ab155987</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with heat shock (44&deg;C) at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa,216 kDa,23 kDa,28 kDa,286 kDa

Observed band size: 15 kDa,260 kDa,48 kDa

false

Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)
  • Dot

Lab

Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] - BSA and Azide free (AB249232)

This data was developed using ab155987, the same antibody clone in a different buffer formulation.

Dot blot analysis of Lane 1 : Hsp27 (pS82) phospho peptide and Lane 2 : Hsp27 non-phospho peptide, labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time : 10 seconds.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7278

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249232 is the carrier-free version of ab155987.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1 phospho S82
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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