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AB17938

Anti-Hsp27 (phospho S86) antibody

5

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal Hsp27 phospho S86 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

Hsp25, Hsp27, Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, Heat shock 27 kDa protein, p25, HSP 25, HSP 27

2 Images
Immunocytochemistry - Anti-Hsp27 (phospho S86) antibody (AB17938)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Hsp27 (phospho S86) antibody (AB17938)

HeLa cells stained for Hsp27 (green) using ab17938 at 1/250 dilution in ICC/IF. It was followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.

Western blot - Anti-Hsp27 (phospho S86) antibody (AB17938)
  • WB

Unknown

Western blot - Anti-Hsp27 (phospho S86) antibody (AB17938)

Hsp25 (phospho S86) antibody image 6534.

Western blot using ab17938 on NIH3T3 cells treated with anisomycin.
Lane 1 : unstimulated cells
Lane 2 : cells stimulated with anisomycin
Lane 3 : cells stimulated with anisomycin. Antibody blocked with the non-phosphopeptide corresponding to the immunogen
Lane 4 : cells stimulated with anisomycin. Antibody blocked with generic phosphoserine-containing peptide
Lane 5 : cells stimulated with anisomycin. Antibody blocked with the phosphopeptide immunogen
Lane 6 : cells stimulated with anisomycin and treated with lambda phosphatase
10-30 μg of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with ab17938 for one hour at room temperature in 3% BSA-TBST buffer, following prior incubation with blocking

All lanes:

Western blot - Anti-Hsp27 (phospho S86) antibody (ab17938)

Predicted band size: 23 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Lysates prepared from NIH3T3 cells were immunoblotted in the presence of non-phosphopeptide corresponding to the immunogen, a generic phosphoserine-containing peptide or the phosphopeptide immunogen. The data show that only the peptide corresponding to the mouse phospho S86 HSP27 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The signal was completely removed by lambda phosphatase treatment demonstrating that the antibody interacts specifically with the phosphorylated protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/250", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated HSP25 (the mouse homolog of human HSP27). The final product is generated by affinity chromatography using an HSP25-derived peptide that is phosphorylated at serine 86.
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization (PubMed : 17661394). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (By similarity).
See full target information Heat shock protein beta-1 phospho S86
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 24:22 PubMed30915129

2019

MiR-214 sensitizes human colon cancer cells to 5-FU by targeting Hsp27.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yang,Yan Bao,Guo-Kai Yang,Jia Wan,Ling-Juan Du,Zhen-Huan Ma

International journal of oncology 49:1839-1847 PubMed27600495

2016

Heat shock protein 27 is a potential indicator for response to YangZheng XiaoJi and chemotherapy agents in cancer cells.

Applications

Unspecified application

Species

Human

Sioned Owen,Huishan Zhao,Alwyn Dart,Yamei Wang,Fiona Ruge,Yong Gao,Cong Wei,Yiling Wu,Wen G Jiang

Developmental cell 17:142-9 PubMed19619499

2009

p38MAPK controls expression of multiple cell cycle inhibitors and islet proliferation with advancing age.

Applications

WB

Species

Mouse

Esther Sook Miin Wong,Xavier Le Guezennec,Oleg N Demidov,Nicolette Theresa Marshall,Siew Tein Wang,Janakiraman Krishnamurthy,Norman E Sharpless,N Ray Dunn,Dmitry V Bulavin
View all publications
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