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AB74442

Anti-Hsp40 antibody [2A7.H6]

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(2 Publications)

Mouse Monoclonal MAS5 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 2 publications. Immunogen corresponding to Full Length Protein corresponding to Saccharomyces cerevisiae S288C YDJ1.

View Alternative Names

MAS5, YNL064C, N2418, YNL2418C, YDJ1, Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1

1 Images
Western blot - Anti-Hsp40 antibody [2A7.H6] (AB74442)
  • WB

Supplier Data

Western blot - Anti-Hsp40 antibody [2A7.H6] (AB74442)

Block : 1.5% BSA for 30 minutes at room temperature.

All lanes:

Western blot - Anti-Hsp40 antibody [2A7.H6] (ab74442) at 1/1000 dilution

All lanes:

Saccharomyces cerevisiae recombinant cell lysate at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG: HRP

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A7.H6

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

Full Length Protein corresponding to Saccharomyces cerevisiae S288C YDJ1. The exact immunogen used to generate this antibody is proprietary information.

P25491

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp40 also known as DnaJB1 is a member of the heat shock protein family that acts mechanically as a molecular chaperone. This protein with a mass of approximately 41 kDa assists in protein folding and stabilization preventing aggregation under stress conditions. Hsp40 proteins are widely expressed in various tissues and are important in maintaining cellular homeostasis. In addition to DnaJB1 other aliases include 3b9 and 2a7 which emphasize its variety within the Hsp40 family.
Biological function summary

Hsp40 proteins participate in cell signaling and stress response by modulating the activity of Hsp70 forming a functional complex with it to guide protein folding processes. This process is essential for protein quality control and cellular protection. It interacts with unfolded proteins and delivers them to Hsp70 enhancing its ATPase activity necessary for the proper functioning of these molecular chaperones. The expression of Hsp40 in stress conditions highlights its importance in the protein quality control system.

Pathways

Hsp40 is involved in important cellular mechanisms like the unfolded protein response and protein catabolism pathways. It works closely with Hsp70 playing a pivotal role in managing protein misfolding and aggregation vital for cellular stress response and recovery. In these pathways Hsp40's function ensures the proper folding and degradation of damaged proteins maintaining proteostasis in the cell.

Hsp40 influences the development of neurodegenerative diseases and certain cancers. Mutations or altered expression of Hsp40 can lead to disrupted protein homeostasis contributing to conditions like Alzheimer's disease and tumor progression. In Alzheimer's disease besides interacting with Hsp70 Hsp40 affects the accumulation of amyloid-beta peptides. In cancer contexts it may interact with other chaperone proteins affecting their function and therefore promoting tumor aggressiveness and resistance to stress.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.
See full target information YDJ1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell stress & chaperones 23:581-594 PubMed29214607

2017

Generation and propagation of yeast prion [URE3] are elevated under electromagnetic field.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yong Lian,Kang-Wei Lin,Chuanjun Yang,Peng Cai

The Journal of cell biology 216:2295-2304 PubMed28630146

2017

ER stress causes widespread protein aggregation and prion formation.

Applications

Unspecified application

Species

Unspecified reactive species

Norfadilah Hamdan,Paraskevi Kritsiligkou,Chris M Grant
View all publications
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Product promise

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