Anti-Hsp40 antibody [3B9.E6]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [3B9.E6] (AB223607)

100% methanol fixed HaCat (human keratinocyte cell line) cells stained for Hsp40 (green) using ab223607 (1/100) in ICC/IF. Secondary Antibody : FITC Goat Anti-Mouse 1/50.

• WB

Lab

Western blot - Anti-Hsp40 antibody [3B9.E6] (AB223607)

Lanes 1-4 : Merged signal (red and green). Green - ab223607 observed at 39 kDa. Red - loading control ab52901 observed at kDa.

ab223607 Anti-Hsp40 antibody [3B9.E6] was shown to specifically react with Hsp40 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266344 (knockout cell lysate ab258400) was used. Wild-type and Hsp40 knockout samples were subjected to SDS-PAGE. ab223607 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DNAJB1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human DNAJB1 (Hsp40) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dnajb1-hsp40-knockout-hek-293t-cell-line-ab266344'>ab266344</a>)

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 38 kDa

Observed band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-Hsp40 antibody [3B9.E6] (AB223607)

All lanes:

Western blot - Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/1000 dilution

Lane 1:

A431 cell lysate at 15 µg

Lane 2:

A549 cell lysate at 15 µg

Lane 3:

HCT116 cell lysate at 15 µg

Lane 4:

HeLa cell lysate at 15 µg

Lane 5:

HEK293 cell lysate at 15 µg

Lane 6:

HepG2 cell lysate at 15 µg

Lane 7:

HL-60 cell lysate at 15 µg

Lane 8:

HUVEC cell lysate at 15 µg

Lane 9:

Jurkat cell lysate at 15 µg

Lane 10:

MCF7 cell lysate at 15 µg

Lane 11:

PC-3 cell lysate at 15 µg

Lane 12:

T98G cell lysate at 15 µg

Lane 13:

Rat brain lysate at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG HRP

Predicted band size: 38 kDa

false