JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB223610

Anti-Hsp40 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Hsp40 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DNAJB1.

View Alternative Names

DNAJ1, HDJ1, HSPF1, DNAJB1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, Human DnaJ protein 1, HSP40, Heat shock protein 40, hDj-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody (AB223610)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody (AB223610)

2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp40 (red) using ab223610 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit APC at 1/200 for 2 hours at room temperature. Counterstain : DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature. Heat shocked at 42°C for 1 hour.

(A) DAPI (blue) nuclear stain (B) ab223610 (C) Merge.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody (AB223610)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody (AB223610)

2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp40 (green) using ab223610 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain : DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature. Heat shocked at 42°C for 1 hour.

(A) DAPI (blue) nuclear stain (B) ab223610 (C) Merge.

Western blot - Anti-Hsp40 antibody (AB223610)
  • WB

Supplier Data

Western blot - Anti-Hsp40 antibody (AB223610)

All lanes:

Western blot - Anti-Hsp40 antibody (ab223610) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Predicted band size: 38 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DNAJB1.

P25685

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp40 also known as DnaJB1 is a member of the heat shock protein family that acts mechanically as a molecular chaperone. This protein with a mass of approximately 41 kDa assists in protein folding and stabilization preventing aggregation under stress conditions. Hsp40 proteins are widely expressed in various tissues and are important in maintaining cellular homeostasis. In addition to DnaJB1 other aliases include 3b9 and 2a7 which emphasize its variety within the Hsp40 family.
Biological function summary

Hsp40 proteins participate in cell signaling and stress response by modulating the activity of Hsp70 forming a functional complex with it to guide protein folding processes. This process is essential for protein quality control and cellular protection. It interacts with unfolded proteins and delivers them to Hsp70 enhancing its ATPase activity necessary for the proper functioning of these molecular chaperones. The expression of Hsp40 in stress conditions highlights its importance in the protein quality control system.

Pathways

Hsp40 is involved in important cellular mechanisms like the unfolded protein response and protein catabolism pathways. It works closely with Hsp70 playing a pivotal role in managing protein misfolding and aggregation vital for cellular stress response and recovery. In these pathways Hsp40's function ensures the proper folding and degradation of damaged proteins maintaining proteostasis in the cell.

Hsp40 influences the development of neurodegenerative diseases and certain cancers. Mutations or altered expression of Hsp40 can lead to disrupted protein homeostasis contributing to conditions like Alzheimer's disease and tumor progression. In Alzheimer's disease besides interacting with Hsp70 Hsp40 affects the accumulation of amyloid-beta peptides. In cancer contexts it may interact with other chaperone proteins affecting their function and therefore promoting tumor aggressiveness and resistance to stress.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed : 9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed : 24318877).
See full target information DNAJB1
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB223607

Anti-Hsp40 antibody [3B9.E6]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [3B9.E6] (AB223607)

  • 1
  • 1
Primary Antibodies

AB74442

Anti-Hsp40 antibody [2A7.H6]

Western blot - Anti-Hsp40 antibody [2A7.H6] (AB74442)

  • 0
  • 0
Primary Antibodies

AB69402

Anti-Hsp40 antibody

Western blot - Anti-Hsp40 antibody (AB69402)

  • 4
  • 5
Primary Antibodies

AB231577

Anti-Hsp40 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp40 antibody (AB231577)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB203085

Anti-Hsp90 beta antibody [EPR16621]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] (AB203085)

  • 0
  • 0
Primary Antibodies

AB181983

Anti-Cyclophilin 40 antibody [EPR14845(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody [EPR14845(B)] (AB181983)

  • 0
  • 0
Primary Antibodies

AB23738

Anti-FOXA1 antibody

KO Validated

Western blot - Anti-FOXA1 antibody (AB23738)

  • 4
  • 11
Primary Antibodies

AB181606

Anti-Hsp70 antibody [EPR16892]

RabMAb|Recombinant|Lab Essentials|Trial Size

Flow Cytometry (Intracellular) - Anti-Hsp70 antibody [EPR16892] (AB181606)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com