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AB69402

Anti-Hsp40 antibody

4

(5 Reviews)

|

(17 Publications)

Rabbit Polyclonal Hsp40 antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples. Cited in 17 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DNAJB1.

View Alternative Names

DNAJ1, HDJ1, HSPF1, DNAJB1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, Human DnaJ protein 1, HSP40, Heat shock protein 40, hDj-1

1 Images
Western blot - Anti-Hsp40 antibody (AB69402)
  • WB

Unknown

Western blot - Anti-Hsp40 antibody (AB69402)

All lanes:

Western blot - Anti-Hsp40 antibody (ab69402) at 1/1000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Hsp40 recombinant protein

Lane 3:

DnaJ recombinant protein

Lane 4:

Cell lysates prepared from heat shocked hela cells

Lane 5:

Cell lysates prepared from heat shocked 3T3 cells

Lane 6:

Cell lysates prepared from PC-12 cells

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DNAJB1.

P25685

Specificity

This antibody detects a ~40 kDa protein, corresponding to the apparent molecular mass of Hsp40 on SDS-PAGE immunoblots. It does not detect DnaJ in E.coli lysates.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" }, "Escherichia coli": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp40 also known as DnaJB1 is a member of the heat shock protein family that acts mechanically as a molecular chaperone. This protein with a mass of approximately 41 kDa assists in protein folding and stabilization preventing aggregation under stress conditions. Hsp40 proteins are widely expressed in various tissues and are important in maintaining cellular homeostasis. In addition to DnaJB1 other aliases include 3b9 and 2a7 which emphasize its variety within the Hsp40 family.
Biological function summary

Hsp40 proteins participate in cell signaling and stress response by modulating the activity of Hsp70 forming a functional complex with it to guide protein folding processes. This process is essential for protein quality control and cellular protection. It interacts with unfolded proteins and delivers them to Hsp70 enhancing its ATPase activity necessary for the proper functioning of these molecular chaperones. The expression of Hsp40 in stress conditions highlights its importance in the protein quality control system.

Pathways

Hsp40 is involved in important cellular mechanisms like the unfolded protein response and protein catabolism pathways. It works closely with Hsp70 playing a pivotal role in managing protein misfolding and aggregation vital for cellular stress response and recovery. In these pathways Hsp40's function ensures the proper folding and degradation of damaged proteins maintaining proteostasis in the cell.

Hsp40 influences the development of neurodegenerative diseases and certain cancers. Mutations or altered expression of Hsp40 can lead to disrupted protein homeostasis contributing to conditions like Alzheimer's disease and tumor progression. In Alzheimer's disease besides interacting with Hsp70 Hsp40 affects the accumulation of amyloid-beta peptides. In cancer contexts it may interact with other chaperone proteins affecting their function and therefore promoting tumor aggressiveness and resistance to stress.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with HSP70 and can stimulate its ATPase activity. Acts also with TTC1 as a chaperone adapter that regulates HSP70-dependent folding process by interacting with the HSP70 amino terminal region (PubMed : 14503850). Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed : 9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed : 24318877).
See full target information DNAJB1
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38892076

2024

Anti-HMGB1 mAb Therapy Reduces Epidural Hematoma Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shangze Gao,Dengli Wang,Keyue Liu,Yasuko Tomono,Li Fu,Yuan Gao,Yohei Takahashi,Mariko Yata,Masahiro Nishibori

Scientific reports 13:8427 PubMed37225872

2023

Alpha-lipoic acid attenuates heat stress-induced apoptosis via upregulating the heat shock response in porcine parthenotes.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hee Lee,Ming-Hong Sun,Wen-Jie Jiang,Xiao-Han Li,Geun Heo,Dongjie Zhou,Zhi Chen,Xiang-Shun Cui

Cancers 14: PubMed36551548

2022

Phosphorylation-Mediated Activation of β-Catenin-TCF4-CEGRs/ALCDs Pathway Is an Essential Event in Development of Aggressive Hepatoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhi Gulati,Margaret A Hanlon,Maggie Lutz,Tyler Quitmeyer,James Geller,Gregory Tiao,Lubov Timchenko,Nikolai Timchenko

Hepatology communications 6:2950-2963 PubMed36000549

2022

β-catenin cancer-enhancing genomic regions axis is involved in the development of fibrolamellar hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhi Gulati,Michael Johnston,Maria Rivas,Ashley Cast,Meenasri Kumbaji,Margaret A Hanlon,Sanghoon Lee,Ping Zhou,Charissa Lake,Emily Schepers,Kyung-Won Min,Je-Hyun Yoon,Rebekah Karns,Lola M Reid,Dolores Lopez-Terrada,Lubov Timchenko,Sreeja Parameswaran,Matthew T Weirauch,Sarangarajan Ranganathan,Alexander Bondoc,James Geller,Gregory Tiao,Soona Shin,Nikolai Timchenko

The Journal of biological chemistry 298:102158 PubMed35724963

2022

A biosensor of protein foldedness identifies increased "holdase" activity of chaperones in the nucleus following increased cytosolic protein aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Candice B Raeburn,Angelique R Ormsby,Dezerae Cox,Chloe A Gerak,Christian Makhoul,Nagaraj S Moily,Simon Ebbinghaus,Alex Dickson,Gawain McColl,Danny M Hatters

The Journal of clinical investigation 131: PubMed33393491

2021

Mitigating oxygen stress enhances aged mouse hematopoietic stem cell numbers and function.

Applications

Unspecified application

Species

Unspecified reactive species

Maegan L Capitano,Safa F Mohamad,Scott Cooper,Bin Guo,Xinxin Huang,Andrea M Gunawan,Carol Sampson,James Ropa,Edward F Srour,Christie M Orschell,Hal E Broxmeyer

Virologica Sinica 35:143-155 PubMed31429011

2019

Proteomic Profiling of Purified Rabies Virus Particles.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Yuyang Wang,Ye Feng,Zhongzhong Tu,Zhiyong Lou,Changchun Tu

Journal of virology 93: PubMed30996089

2019

Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Fan Yang,Zixiang Zhu,Yang Yang,Zhifang Wang,Weijun Cao,Wen Dang,Linlin Li,Ruoqing Mao,Yongjie Liu,Hong Tian,Keshan Zhang,Xiangtao Liu,Junwu Ma,Haixue Zheng

Journal of molecular biology 431:2612-2627 PubMed31082436

2019

Beta-sheet-specific interactions with heat shock proteins define a mechanism of delayed tumor cell death in response to HAMLET.

Applications

Unspecified application

Species

Unspecified reactive species

Aftab Nadeem,James C S Ho,Tuan Hiep Tran,Sanchari Paul,Victoria Granqvist,Nadege Despretz,Catharina Svanborg

EMBO reports 19: PubMed29420235

2018

Loss of mitochondrial protease ClpP protects mice from diet-induced obesity and insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Shylesh Bhaskaran,Gavin Pharaoh,Rojina Ranjit,Ashley Murphy,Satoshi Matsuzaki,Binoj C Nair,Brittany Forbes,Suzana Gispert,Georg Auburger,Kenneth M Humphries,Michael Kinter,Timothy M Griffin,Sathyaseelan S Deepa
View all publications
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Product promise

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