Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)

Knockout Tested Rabbit Recombinant Monoclonal Hsp40 antibody. Carrier free. Suitable for WB, ICC/IF, IP, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Western blot - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (AB315993), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IP	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Tested	Tested
Rat	Tested	Tested	Expected	Tested	Tested
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information DNAJB1

Function

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).

Alternative names

DNAJ1, HDJ1, HSPF1, DNAJB1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, Human DnaJ protein 1, HSP40, Heat shock protein 40, hDj-1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25331-69
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315993 is the carrier-free version of Anti-Hsp40 antibody [EPR25331-69] ab315992.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp40 also known as DnaJB1 is a member of the heat shock protein family that acts mechanically as a molecular chaperone. This protein with a mass of approximately 41 kDa assists in protein folding and stabilization preventing aggregation under stress conditions. Hsp40 proteins are widely expressed in various tissues and are important in maintaining cellular homeostasis. In addition to DnaJB1 other aliases include 3b9 and 2a7 which emphasize its variety within the Hsp40 family.

Biological function summary

Hsp40 proteins participate in cell signaling and stress response by modulating the activity of Hsp70 forming a functional complex with it to guide protein folding processes. This process is essential for protein quality control and cellular protection. It interacts with unfolded proteins and delivers them to Hsp70 enhancing its ATPase activity necessary for the proper functioning of these molecular chaperones. The expression of Hsp40 in stress conditions highlights its importance in the protein quality control system.

Pathways

Hsp40 is involved in important cellular mechanisms like the unfolded protein response and protein catabolism pathways. It works closely with Hsp70 playing a pivotal role in managing protein misfolding and aggregation vital for cellular stress response and recovery. In these pathways Hsp40's function ensures the proper folding and degradation of damaged proteins maintaining proteostasis in the cell.

Associated diseases and disorders

Hsp40 influences the development of neurodegenerative diseases and certain cancers. Mutations or altered expression of Hsp40 can lead to disrupted protein homeostasis contributing to conditions like Alzheimer's disease and tumor progression. In Alzheimer's disease besides interacting with Hsp70 Hsp40 affects the accumulation of amyloid-beta peptides. In cancer contexts it may interact with other chaperone proteins affecting their function and therefore promoting tumor aggressiveness and resistance to stress.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

Western blot - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
This antibody does not cross-react with human DNAJB5 (Hsp40 member 5) and DNAJB4 (Hsp40 member 4).
All lanes: Western blot - Anti-Hsp40 antibody [EPR25331-69] (Anti-Hsp40 antibody [EPR25331-69] ab315992) at 1/1000 dilution
Lane 1: His-tagged human DNAJB1 (Hsp40) recombinant protein at 10 ng with 5% NFDM/TBST
Lane 2: His-tagged human DNAJB5 (Hsp40 member 5) recombinant protein at 20 ng with 5% NFDM/TBST
Lane 3: His-tagged human DNAJB4 (Hsp40 member 4) recombinant protein at 40 ng with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 38 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon. The section was incubated with Anti-Hsp40 antibody [EPR25331-69] ab315992 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cervical cancer. The section was incubated with Anti-Hsp40 antibody [EPR25331-69] ab315992 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon. The section was incubated with Anti-Hsp40 antibody [EPR25331-69] ab315992 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat colon. The section was incubated with Anti-Hsp40 antibody [EPR25331-69] ab315992 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK-293T (human epithelial cell line from embryonic kidney) cell pellet (B) DNAJB1 (Hsp40) knockout HEK-293T cell pellet tissue labeling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/5000 (0.099 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) Wild-type HEK-293T (human epithelial cell line from embryonic kidney) cell pellet, no staining on (B) DNAJB1 (Hsp40) knockout HEK-293T cell pellet. The section was incubated with Anti-Hsp40 antibody [EPR25331-69] ab315992 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Hsp40 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Lane 2: Anti-Hsp40 antibody [EPR25331-69] ab315992 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Hsp40 antibody [EPR25331-69] ab315992 in HEK-293 whole cell lysate
All lanes: Immunoprecipitation - Anti-Hsp40 antibody [EPR25331-69] (Anti-Hsp40 antibody [EPR25331-69] ab315992) at 1/30 dilution
All lanes: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 32s
Western blot - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Hsp40 antibody [EPR25331-69] ab315992 was shown to bind specifically to DNAJB1 (Hsp40). Target of interest was observed at 38 kDa in wild-type HEK-293 cell lysates (lane 1) with no signal observed at this size in DNAJB1 (Hsp40) knockout cell line (lane 2, knockout cell line Human DNAJB1 (Hsp40) knockout HEK-293T cell line ab266344/ knockout cell lysate Human DNAJB1 (Hsp40) knockout HEK-293T cell lysate ab258400).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Hsp40 antibody [EPR25331-69] (Anti-Hsp40 antibody [EPR25331-69] ab315992) at 1/1000 dilution
Lane 1: Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: DNAJB1 (Hsp40) knockout HEK-293 whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 5: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 38 kDa, 124 kDa
Exposure time: 70s
Western blot - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
The identity of the higher MW band at approximately 120 kDa (in lane 2) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Hsp40 antibody [EPR25331-69] (Anti-Hsp40 antibody [EPR25331-69] ab315992) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse colon tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Rat testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Rat colon tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 38 kDa, 36 kDa
Exposure time: 70s
Western blot - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
The lanes 1 and 4 were developed using a high sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 59 seconds, lane 2: 70 seconds, lanes 3-4: 180 seconds.
All lanes: Western blot - Anti-Hsp40 antibody [EPR25331-69] (Anti-Hsp40 antibody [EPR25331-69] ab315992) at 1/1000 dilution
Lane 1: Human colon cancer tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Human colon tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 38 kDa, 36 kDa
Flow Cytometry (Intracellular) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/500 dilution (0.1 ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/500 dilution (0.1 ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized DNAJB1 (Hsp40) KO HEK293T (DNAJB1 (Hsp40) knockout human embryonic kidney epithelial cell, Left) / Parental HEK293T (Right) cells labelling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/500 dilution (0.1 ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized DNAJB1 (Hsp40) KO HEK293T ( DNAJB1 (Hsp40) knockout human embryonic kidney epithelial cell) (Human DNAJB1 (Hsp40) knockout HEK-293T cell line ab266344) cells labelling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/100 (4.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear and cytoplasmic staining in parental HEK293T cells and no staining in DNAJB1 (Hsp40) KO HEK293T cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/100 (4.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear and cytoplasmic staining in C6 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [EPR25331-69] - BSA and Azide free (ab315993)
This data was developed using Anti-Hsp40 antibody [EPR25331-69] ab315992, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Hsp40 with Anti-Hsp40 antibody [EPR25331-69] ab315992 at 1/100 (4.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear and cytoplasmic staining in NIH/3T3 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.