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AB242006

Anti-Hsp47 antibody [1C4-1A6]

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(1 Publication)

Mouse Monoclonal Hsp47 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SERPINH1.

View Alternative Names

CBP1, CBP2, HSP47, SERPINH2, PIG14, SERPINH1, Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Rheumatoid arthritis-related antigen RA-A47, AsTP3, Colligin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)

2% paraformaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp47 (green, B) using ab242006 at 1/100 dilution in ICC/IF. The nuclear counter stain is DAPI (blue, A). Panel C shows the merged images.

Primary incubation for 12 hours at 4°.

Secondary antibody : FITC Goat Anti-Mouse (green) at 1 : 200 for 2 hours at RT.

Cell were heat shocked at 42°C for 1 hour.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp47 antibody [1C4-1A6] (AB242006)

2% paraformaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp47 (red, B) using ab242006 at 1/100 dilution in ICC/IF. The nuclear counter stain is DAPI (blue, A). Panel C shows the merged images.

Primary incubation for 12 hours at 4°.

Secondary antibody : APC Goat Anti-Mouse (red) at 1 : 200 for 2 hours at RT.

Cell were heat shocked at 42°C for 1 hour.

Western blot - Anti-Hsp47 antibody [1C4-1A6] (AB242006)
  • WB

Supplier Data

Western blot - Anti-Hsp47 antibody [1C4-1A6] (AB242006)

Primary incubation for 1 hour at room temperature.

Block : 5% milk + TBST for 1 hour at room temperature.

Lane 1:

Western blot - Anti-Hsp47 antibody [1C4-1A6] (ab242006) at 1/250 dilution

Lane 2:

Western blot - Anti-Hsp47 antibody [1C4-1A6] (ab242006) at 1/500 dilution

Lane 3:

Western blot - Anti-Hsp47 antibody [1C4-1A6] (ab242006) at 1/1000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

HRP Goat Anti-Mouse at 1/50 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C4-1A6

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SERPINH1.

P50454

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp47 also known as the heat shock protein 47 is a molecular chaperone with a molecular weight of approximately 47 kDa. It mainly resides in the endoplasmic reticulum and participates in collagen synthesis. Hsp47 binds specifically to unfolded procollagen chains ensures their proper folding and prevents premature aggregation. Due to its specialized function Hsp47 is highly expressed in collagen-producing cells like fibroblasts. Researchers commonly study this protein using techniques like Hsp47 ELISA to measure its expression or activity levels.
Biological function summary

Hsp47 ensures the quality control of collagen within the secretory pathway. It does not form part of any large protein complexes but operates closely with collagen molecules. By stabilizing procollagen's triple-helix structure Hsp47 plays an important role in collagen biosynthesis. Dysfunctional collagen processes can arise without this protein's presence potentially leading to cellular stress or structural weaknesses within tissues rich in collagen.

Pathways

Hsp47's activity integrates into the collagen synthesis and secretion pathways. This protein is related to other molecular chaperones such as Hsp70 that assist in maintaining protein homeostasis within the cell. Hsp47’s function links to fibrosis pathways because of its role in collagen maturation. In terms of fibrotic pathologies this involvement makes the chaperoning of procollagen a critical control point.

Disrupted Hsp47 function frequently associates with fibrotic conditions and osteogenesis imperfecta. Fibrosis results from excessive collagen deposition in which Hsp47 is deeply implicated. Additionally certain cancer types have shown altered Hsp47 expression correlating with increased cancer cell invasiveness due to extracellular matrix remodeling. Studies have noted interactions between Hsp47 and TGF-beta signaling in fibrotic tissues reinforcing its role in pathological collagen turnover.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
See full target information SERPINH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 23:e14209 PubMed38825816

2024

Sevoflurane-induced overexpression of extrasynaptic α5-GABAR via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhun Wang,Jinpeng Dong,Mengxue Zhang,Sixuan Wang,Jiangnan Wu,Shengran Wang,Yuan Luo,Yongan Wang,Yiqing Yin
View all publications
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Product promise

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