Anti-Hsp60 antibody [24/HSP60] (BSA and Azide free)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (BSA and Azide free) (AB300660)

This data was developed using ab300659, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Hsp60 with ab300659 at 1/250 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line is observed. ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (BSA and Azide free) (AB300660)

This data was developed using ab300659, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Hsp60 with ab300659 at 1/250 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in RAW 264.7 cell line is observed. ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (BSA and Azide free) (AB300660)

This data was developed using ab300659, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling Hsp60 with ab300659 at 1/250 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in C6 cell line is observed. ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/200 10 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-Hsp60 antibody [24/HSP60] (BSA and Azide free) (AB300660)

All lanes:

Western blot - Anti-Hsp60 antibody [24/HSP60] - Mitochondrial Marker (<a href='/en-us/products/primary-antibodies/hsp60-antibody-24-hsp60-mitochondrial-marker-ab300659'>ab300659</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 7:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 8:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 61 kDa

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