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Rabbit Polyclonal Hsp60 antibody. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human, Mouse, Pig, Rat samples. Cited in 131 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (AB46798), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Hsp60 antibody (AB46798), expandable thumbnail
  • Immunoprecipitation - Anti-Hsp60 antibody (AB46798), expandable thumbnail
  • Western blot - Anti-Hsp60 antibody (AB46798), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (AB46798), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)WBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Tested
Expected
Rat
Expected
Expected
Expected
Tested
Expected
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Predicted
Hamster
Predicted
Predicted
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted
Predicted
Predicted
Pig
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/40

Notes

-

Expected
Expected

Species

Mouse, Pig, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Hamster, Cow, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Pig, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Hamster, Cow, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/20000

Notes

-

Species

Pig

Dilution info

1/20000

Notes

-

Species

Human

Dilution info

1/20000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Hamster, Cow, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Hamster, Cow, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5.00000-10.00000 µg/mL

Notes

-

Expected
Expected

Species

Mouse, Pig, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Hamster, Cow, Orangutan

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

Alternative names

Recommended products

Rabbit Polyclonal Hsp60 antibody. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human, Mouse, Pig, Rat samples. Cited in 131 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

Replenishment batches of our polyclonal antibody, ab46798 are tested in WB. Previous batches were additionally validated in Flow Cyt, ICC/IF, IHC-P and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab190828.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail
    This image is courtesy of a customer review submitted by Nicole Schechter Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Unpurified ab46798 staining Hsp60 from human colon tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 594 goat polyclonal, diluted 1/5000, was used as the secondary.

  • Flow Cytometry (Intracellular) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Hsp60 antibody (ab46798)

    Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab46798 at a dilution of 1 in 150 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black) and cells without incubation with the antibody were used as a negative control (blue line).

  • Immunoprecipitation - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunoprecipitation - Anti-Hsp60 antibody (ab46798)

    ab46798 (purified) at 1/40 immunoprecipitating Hsp60 in MCF7 (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-Hsp60 antibody (ab46798)

    Predicted band size: 61 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Western blot - Anti-Hsp60 antibody (ab46798)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp60 antibody (ab46798) at 1/20000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Rat heart tissue lysate at 10 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded human lung adenocarcinoma with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Western blot - Anti-Hsp60 antibody (ab46798)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp60 antibody (ab46798) at 1/20000 dilution

    All lanes: T47-D cell lysate at 10 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Western blot - Anti-Hsp60 antibody (ab46798)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp60 antibody (ab46798) at 1/50000 dilution

    Lane 1: MCF7 cell lysate at 20 µg

    Lane 2: SW480 cell lysate at 20 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded rat kidney with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using unpurified ab46798 at 1/250 dilution.

  • Western blot - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Western blot - Anti-Hsp60 antibody (ab46798)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Hsp60 antibody (ab46798) at 1/50000 dilution

    All lanes: Pig heart tissue lysate at 20 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Western blot - Anti-Hsp60 antibody (ab46798)

    Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

    All lanes: Western blot - Anti-Hsp60 antibody (ab46798) at 1/50000 dilution

    All lanes: Western blot - T-47D whole cell lysate (T-47D whole cell lysate ab14899) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/2000 dilution

    Predicted band size: 61 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    IHC image of Hsp60 staining in a section of formalin-fixed paraffin-embedded normal human liver* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab46798, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)

    ab46798 staining Hsp60 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab46798 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry - Anti-Hsp60 antibody (ab46798), expandable thumbnail
    This image is courtesy of a customer review submitted by Suleyman Bozkurt

    Immunocytochemistry - Anti-Hsp60 antibody (ab46798)

    Immunocytochemistry analysis of paraformaldehyde-fixed 0.3% Triton X permeabilized HeLa FlipIn Cell line staining with ab46798 at 1/200 dilution. Secondary antibody was Alexa fluor™ 488 goat anti-rabbit IgG H+L at 1/700 dilution. Samples were incubated with the primary antibody with blocking buffer (5% FBS in PBS) for 2 hours at 24°C. Blocking was done using 5% serum for 1 hour at 24°C.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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