AB59458

Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker

Name: Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458) | Abcam
Brand: Abcam Limited
SKU: AB59458
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(6 Publications)

Mouse Monoclonal Hsp60 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPD1.

View Alternative Names

HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

All lanes:

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458) at 1/1000 dilution

All lanes:

HeLa cell lysate

Predicted band size: 61 kDa

false

AbReview42300****

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Lanes 1 - 3:

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458) at 1/4000 dilution

Lane 4:

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458)

Lanes 1 - 3:

Saccharomyces cerevisiae whole cell lysate at 6900000 Cells

Lane 4:

Ladder

Secondary

Lanes 1 - 2:

HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution

Lane 3:

HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 25s

This image is courtesy of an anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LK-2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HSPD1.

P10809

Specificity

Detects ~60kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/40000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp60 also known as HSPD1 and heat shock protein 60 is a significant chaperonin with a molecular weight of approximately 60 kDa. It resides predominantly in the mitochondria and functions to assist in the proper folding of proteins preventing their aggregation. Hsp60 is expressed mainly in cells with high metabolic activity. Its presence as a mitochondrial marker highlights its essential role in maintaining cellular homeostasis and function.

Biological function summary

The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.

Pathways

Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.

Hsp60 shows a connection to neurodegenerative diseases and cancer. Altered Hsp60 levels correlate with increased apoptosis in neurodegenerative conditions like Alzheimer's disease. Additionally in cancer interactions with proteins such as AKT suggest its potential role in cell proliferation and survival. Understanding its role could aid in the development of therapeutic interventions targeting mitochondrial dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed : 11422376, PubMed : 1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

See full target information HSPD1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:4 PubMed33398015

2021

Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N-fixation in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Jiang,Lucía Payá-Tormo,Diana Coroian,Inés García-Rubio,Rocío Castellanos-Rueda,Álvaro Eseverri,Gema López-Torrejón,Stefan Burén,Luis Manuel Rubio

Journal of cell science 132: PubMed31615968

2019

Spatial integration of mechanical forces by α-actinin establishes actin network symmetry.

Applications

Unspecified application

Species

Unspecified reactive species

Fabrice Senger,Amandine Pitaval,Hajer Ennomani,Laetitia Kurzawa,Laurent Blanchoin,Manuel Théry

Scientific reports 9:6152 PubMed30992471

2019

Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gianluca Farrugia,Maria Azzopardi,Christian Saliba,Godfrey Grech,Angelina S Gross,Jelena Pistolic,Vladimir Benes,Neville Vassallo,Joseph Borg,Frank Madeo,Tobias Eisenberg,Rena Balzan

Journal of virology 91: PubMed28446677

2017

Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela J Schultz,Sharon Isern,Scott F Michael,Ronald B Corley,John H Connor,Horacio M Frydman

Journal of immunology (Baltimore, Md. : 1950) 193:4568-79 PubMed25267976

2014

Mast cells control the expansion and differentiation of IL-10-competent B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Mion,Federica D'Incà,Luca Danelli,Barbara Toffoletto,Carla Guarnotta,Barbara Frossi,Alessia Burocchi,Alice Rigoni,Norbert Gerdes,Esther Lutgens,Claudio Tripodo,Mario P Colombo,Juan Rivera,Gaetano Vitale,Carlo E Pucillo

Journal of proteome research 8:1131-42 PubMed19226166

2009

Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates.

Applications

Unspecified application

Species

Unspecified reactive species

Nicoletta Bivi,Jessica Z Bereszczak,Milena Romanello,Leo A H Zeef,Daniela Delneri,Franco Quadrifoglio,Luigi Moro,Francesco L Brancia,Gianluca Tell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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