Mouse Monoclonal Hsp60 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPD1.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (AB59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59502.png/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (AB59458)")
![Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--western-blot-img98426.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)")
![Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--western-blot-img138215.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (AB59458)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (AB59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115204.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (AB59458)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (AB59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img5706.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (AB59458)")
Publications
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- Communications biology 4:42021Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N-fixation in plants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Jiang et. al.PubMed 33398015
- Journal of cell science 132:2019Spatial integration of mechanical forces by α-actinin establishes actin network symmetry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabrice Senger et. al.PubMed 31615968
- Scientific reports 9:61522019Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGianluca Farrugia et. al.PubMed 30992471
- Journal of virology 91:2017Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaela J Schultz et. al.PubMed 28446677
- Journal of immunology (Baltimore, Md. : 1950) 193:4568-792014Mast cells control the expansion and differentiation of IL-10-competent B cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Mion et. al.PubMed 25267976
- Journal of proteome research 8:1131-422009Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicoletta Bivi,Jessica Z Bereszczak,Milena Romanello,Leo A H Zeef,Daniela Delneri,Franco Quadrifoglio,Luigi Moro,Francesco L Brancia,Gianluca TellPubMed 19226166
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human HSPD1. Database link P10809
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Alternative names
HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60
Recommended products
Mouse Monoclonal Hsp60 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPD1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human HSPD1. Database link P10809
- Clone number
- LK-2
- Specificity
Detects ~60kDa.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp60 also known as HSPD1 and heat shock protein 60 is a significant chaperonin with a molecular weight of approximately 60 kDa. It resides predominantly in the mitochondria and functions to assist in the proper folding of proteins preventing their aggregation. Hsp60 is expressed mainly in cells with high metabolic activity. Its presence as a mitochondrial marker highlights its essential role in maintaining cellular homeostasis and function.
- Biological function summary
The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.
- Pathways
Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.
- Associated diseases and disorders
Hsp60 shows a connection to neurodegenerative diseases and cancer. Altered Hsp60 levels correlate with increased apoptosis in neurodegenerative conditions like Alzheimer's disease. Additionally in cancer interactions with proteins such as AKT suggest its potential role in cell proliferation and survival. Understanding its role could aid in the development of therapeutic interventions targeting mitochondrial dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59502.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.
![Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--western-blot-img98426.jpg "Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458)")
Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458)
Hsp60 Western blot staining of HeLa cell lysate using mouse Anti-Hsp60 antibody
All lanes: Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458) at 1/1000 dilution
All lanes: HeLa cell lysate
Predicted band size: 61 kDa
![Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--western-blot-img138215.jpg "Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458)")
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458)
Hsp60 Western blot staining using mouse Anti-Hsp60 antibody
Lanes 1 - 3: Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458) at 1/4000 dilution
Lane 4: Western blot - Anti-Hsp60 antibody [LK-2] - Mitochondrial Marker (ab59458)
Lanes 1 - 3: Saccharomyces cerevisiae whole cell lysate at 6900000 Cells
Lane 4: Ladder
SecondaryLanes 1 - 2: HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
Lane 3: HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 60 kDa
Exposure time: 25s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115204.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458), expandable thumbnail](https://content.abcam.com/products/images/hsp60-antibody-lk-2-mitochondrial-marker-ab59458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img5706.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.
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