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Mouse Monoclonal Hsp60 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPD1.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (AB233878), expandable thumbnail
  • Immunocytochemistry - Anti-Hsp60 antibody [LK1] (AB233878), expandable thumbnail
  • Flow Cytometry - Anti-Hsp60 antibody [LK1] (AB233878), expandable thumbnail
  • Western blot - Anti-Hsp60 antibody [LK1] (AB233878), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (AB233878), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human HSPD1. Database link P10809

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytWBIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Bacteria
Not recommended
Not recommended
Not recommended
Not recommended
Chicken
Not recommended
Not recommended
Not recommended
Not recommended
Cow
Not recommended
Not recommended
Not recommended
Not recommended
Dog
Not recommended
Not recommended
Not recommended
Not recommended
Drosophila melanogaster
Not recommended
Not recommended
Not recommended
Not recommended
Hamster
Not recommended
Not recommended
Not recommended
Not recommended
Monkey
Not recommended
Not recommended
Not recommended
Not recommended
Pig
Not recommended
Not recommended
Not recommended
Not recommended
Rabbit
Not recommended
Not recommended
Not recommended
Not recommended
Sheep
Not recommended
Not recommended
Not recommended
Not recommended
Spinacia oleracea
Not recommended
Not recommended
Not recommended
Not recommended
Xenopus laevis
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Not recommended
Not recommended

Species

Bacteria

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Spinacia oleracea

Dilution info

-

Notes

-

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Sheep

Dilution info

-

Notes

-

Species

Rabbit

Dilution info

-

Notes

-

Species

Chicken

Dilution info

-

Notes

-

Species

Hamster

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Dog

Dilution info

-

Notes

-

Species

Pig

Dilution info

-

Notes

-

Species

Xenopus laevis

Dilution info

-

Notes

-

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Species

Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg for 106 Cells

Notes

-

Not recommended
Not recommended

Species

Bacteria, Spinacia oleracea, Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25-0.5 µg/mL

Notes

-

Not recommended
Not recommended

Species

Bacteria, Spinacia oleracea, Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Primary incubation for 30 minutes at RT.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Bacteria

Dilution info

-

Notes

Primary incubation for 30 minutes at RT.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Spinacia oleracea

Dilution info

-

Notes

Primary incubation for 30 minutes at RT.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Sheep

Dilution info

-

Notes

-

Species

Rabbit

Dilution info

-

Notes

-

Species

Chicken

Dilution info

-

Notes

-

Species

Hamster

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Dog

Dilution info

-

Notes

-

Species

Pig

Dilution info

-

Notes

-

Species

Xenopus laevis

Dilution info

-

Notes

-

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Species

Monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

2 products for Alternative Version

Target data

Function

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

Alternative names

Recommended products

Mouse Monoclonal Hsp60 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPD1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human HSPD1. Database link P10809
Clone number

LK1

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp60 also known as HSPD1 and heat shock protein 60 is a significant chaperonin with a molecular weight of approximately 60 kDa. It resides predominantly in the mitochondria and functions to assist in the proper folding of proteins preventing their aggregation. Hsp60 is expressed mainly in cells with high metabolic activity. Its presence as a mitochondrial marker highlights its essential role in maintaining cellular homeostasis and function.

Biological function summary

The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.

Pathways

Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.

Associated diseases and disorders

Hsp60 shows a connection to neurodegenerative diseases and cancer. Altered Hsp60 levels correlate with increased apoptosis in neurodegenerative conditions like Alzheimer's disease. Additionally in cancer interactions with proteins such as AKT suggest its potential role in cell proliferation and survival. Understanding its role could aid in the development of therapeutic interventions targeting mitochondrial dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Hsp60 using ab233878 at 1 μg/ml in immunohistochemical analysis.

  • Immunocytochemistry - Anti-Hsp60 antibody [LK1] (ab233878), expandable thumbnail

    Immunocytochemistry - Anti-Hsp60 antibody [LK1] (ab233878)

    Immunocytochemistry analysis of PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells labeling Hsp60 with ab233878 at 2 μg/mL followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Reddot (red).

  • Flow Cytometry - Anti-Hsp60 antibody [LK1] (ab233878), expandable thumbnail

    Flow Cytometry - Anti-Hsp60 antibody [LK1] (ab233878)

    Flow Cytometry analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp60 with ab233878 at 2 μg/106 cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Western blot - Anti-Hsp60 antibody [LK1] (ab233878), expandable thumbnail

    Western blot - Anti-Hsp60 antibody [LK1] (ab233878)

    All lanes: Western blot - Anti-Hsp60 antibody [LK1] (ab233878) at 0.5 µg/mL

    All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab233878)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for Hsp60 using ab233878 at 1 μg/ml in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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