AB82520

Anti-Hsp60 antibody - Mitochondrial Marker

Lab Essentials
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(3 Publications)

Goat Polyclonal Hsp60 antibody. Suitable for WB and reacts with Recombinant full length protein, Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPD1.

View Alternative Names

HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60

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Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB82520)

All lanes:

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (ab82520) at 1/1000 dilution

Lane 1:

Hsp60 Active Recombinant Protein

Lane 2:

GroEL Active Recombinant Protein

Lane 3:

Hsp65 Protein

Lane 4:

HeLa Cell Lysate

Lane 5:

3T3 Cell Lysate

Lane 6:

RK-13 Cell Lysate

Predicted band size: 61 kDa

Observed band size: 61 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HSPD1.

P10809

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp60 also known as HSPD1 and heat shock protein 60 is a significant chaperonin with a molecular weight of approximately 60 kDa. It resides predominantly in the mitochondria and functions to assist in the proper folding of proteins preventing their aggregation. Hsp60 is expressed mainly in cells with high metabolic activity. Its presence as a mitochondrial marker highlights its essential role in maintaining cellular homeostasis and function.

Biological function summary

The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.

Pathways

Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.

Hsp60 shows a connection to neurodegenerative diseases and cancer. Altered Hsp60 levels correlate with increased apoptosis in neurodegenerative conditions like Alzheimer's disease. Additionally in cancer interactions with proteins such as AKT suggest its potential role in cell proliferation and survival. Understanding its role could aid in the development of therapeutic interventions targeting mitochondrial dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed : 11422376, PubMed : 1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

See full target information HSPD1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114103 PubMed38607920

2024

HIF1α-dependent uncoupling of glycolysis suppresses tumor cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés A Urrutia,Claudia Mesa-Ciller,Andrea Guajardo-Grence,H Furkan Alkan,Inés Soro-Arnáiz,Anke Vandekeere,Ana Margarida Ferreira Campos,Sebastian Igelmann,Lucía Fernández-Arroyo,Gianmarco Rinaldi,Doriane Lorendeau,Katrien De Bock,Sarah-Maria Fendt,Julián Aragonés

Nature protocols 16:5287-5308 PubMed34686857

2021

Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tito Calì,Marisa Brini

Cell death and differentiation 25:1131-1145 PubMed29229997

2017

SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Cieri,Mattia Vicario,Marta Giacomello,Francesca Vallese,Riccardo Filadi,Tina Wagner,Tullio Pozzan,Paola Pizzo,Luca Scorrano,Marisa Brini,Tito Calì

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Hsp60 antibody - Mitochondrial Marker

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB82520)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise