Skip to main content

Rabbit Polyclonal HSPA1B antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSPA1B aa 300 to C-terminus.


Images

Western blot - Anti-Hsp70 antibody (AB137680), expandable thumbnail
  • Western blot - Anti-Hsp70 antibody (AB137680), expandable thumbnail
  • Western blot - Anti-Hsp70 antibody (AB137680), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (AB137680), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HSPA1B aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P0DMV9

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Cow
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Rat

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1 (PubMed:24012426, PubMed:24318877, PubMed:26865365). Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation (PubMed:27708256). Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle (PubMed:27137183). Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling (PubMed:24613385). Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223).(Microbial infection) In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.

Alternative names

Recommended products

Rabbit Polyclonal HSPA1B antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSPA1B aa 300 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HSPA1B aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P0DMV9
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp70 also known as Heat Shock Protein 70 or HSPA1B is a molecular chaperone with a mass of approximately 70 kDa. It plays a mechanical role by assisting in the proper folding of nascent polypeptide chains and the refolding of misfolded proteins. Researchers often detect Hsp70 using Western blot and immunohistochemistry (IHC) techniques. Hsp70 is widely expressed in many tissues particularly during stress conditions like heat shock where its expression level increases significantly.

Biological function summary

Hsp70 operates by stabilizing intermediate states of folding proteins preventing aggregation and facilitating the correct folding process. It often forms a complex with co-chaperones such as Hsp40 and nucleotide exchange factors. This complex is essential for the protein's activity and function. Additionally Hsp70 participates in protein degradation pathways by guiding misfolded proteins to the proteasome for degradation maintaining cellular homeostasis.

Pathways

This molecular chaperone plays significant roles in the heat shock response and unfolded protein response pathways. Hsp70 interacts closely with proteins such as Hsp90 and co-chaperones which together help protect cells from stress-induced damage. The protein also participates in the JAK/STAT signaling pathway influencing cell proliferation and apoptosis. These interactions suggest an integral role in maintaining cellular integrity during stress conditions.

Associated diseases and disorders

Overexpression of Hsp70 has been associated with various cancers and neurodegenerative diseases. In cancer Hsp70 helps tumor cells survive the hostile tumor microenvironment partly by interacting with anti-apoptotic proteins such as Bcl-2. In neurodegenerative disorders such as Alzheimer's disease Hsp70 associates with amyloid-beta peptides potentially mitigating their aggregation toxicity. These interactions highlight Hsp70's importance in both protective and pathological cellular processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Hsp70 antibody (ab137680), expandable thumbnail

    Western blot - Anti-Hsp70 antibody (ab137680)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-Hsp70 antibody (ab137680) at 1/1000 dilution

    Lane 1: 293T whole cell lysate at 30 µg

    Lane 2: A431 whole cell lysate at 30 µg

    Lane 3: H1299 whole cell lysate at 30 µg

    Lane 4: HeLa S3 whole cell lysate at 30 µg

    Lane 5: HepG2 whole cell lysate at 30 µg

    Lane 6: MOLT4 whole cell lysate at 30 µg

    Lane 7: Raji whole cell lysate at 30 µg

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody (ab137680), expandable thumbnail

    Western blot - Anti-Hsp70 antibody (ab137680)

    All lanes: Western blot - Anti-Hsp70 antibody (ab137680) at 1/2500 dilution

    All lanes: Rat brain whole cell lysate at 40 µg

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody (ab137680), expandable thumbnail

    Western blot - Anti-Hsp70 antibody (ab137680)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-Hsp70 antibody (ab137680) at 1/1000 dilution

    All lanes: Mouse brain whole cell lysate at 50 µg

    Predicted band size: 59 kDa, 70 kDa, 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab137680), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab137680)

    Immunofluorescence analysis of methanol-fixed HeLa cells labelling Hsp70 with ab137680 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com