AB240902

Anti-Hsp70 antibody

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(1 Publication)

Rabbit Polyclonal HSPA4 antibody. Suitable for WB and reacts with Trypanosoma brucei samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Trypanosoma brucei brucei Heat shock 70 kDa protein 4 aa 200-300.

View Alternative Names

Heat shock 70 kDa protein 4, HSP70-4

1 Images

Western blot - Anti-Hsp70 antibody (AB240902)

WB

Supplier Data

Western blot - Anti-Hsp70 antibody (AB240902)

Primary incubation for 2 hours at room temperature.

Block : 5% Skim Milk in 1X TBST.

All lanes:

Western blot - Anti-Hsp70 antibody (ab240902) at 1/1000 dilution

Lane 1:

Trypanosoma brucei brucei (cells incubated at 37°C) cell lysate at 15 µg

Lane 2:

Trypanosoma brucei brucei (cells incubated for 1 hour at 42°C) cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG: HRP at 1/3000 dilution

Predicted band size: 70 kDa

true

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Trypanosoma brucei

Applications

WB

Immunogen

Synthetic Peptide within Trypanosoma brucei brucei Heat shock 70 kDa protein 4 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Trypanosoma brucei": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp70 also known as Heat Shock Protein 70 or HSPA1B is a molecular chaperone with a mass of approximately 70 kDa. It plays a mechanical role by assisting in the proper folding of nascent polypeptide chains and the refolding of misfolded proteins. Researchers often detect Hsp70 using Western blot and immunohistochemistry (IHC) techniques. Hsp70 is widely expressed in many tissues particularly during stress conditions like heat shock where its expression level increases significantly.

Biological function summary

Hsp70 operates by stabilizing intermediate states of folding proteins preventing aggregation and facilitating the correct folding process. It often forms a complex with co-chaperones such as Hsp40 and nucleotide exchange factors. This complex is essential for the protein's activity and function. Additionally Hsp70 participates in protein degradation pathways by guiding misfolded proteins to the proteasome for degradation maintaining cellular homeostasis.

Pathways

This molecular chaperone plays significant roles in the heat shock response and unfolded protein response pathways. Hsp70 interacts closely with proteins such as Hsp90 and co-chaperones which together help protect cells from stress-induced damage. The protein also participates in the JAK/STAT signaling pathway influencing cell proliferation and apoptosis. These interactions suggest an integral role in maintaining cellular integrity during stress conditions.

Overexpression of Hsp70 has been associated with various cancers and neurodegenerative diseases. In cancer Hsp70 helps tumor cells survive the hostile tumor microenvironment partly by interacting with anti-apoptotic proteins such as Bcl-2. In neurodegenerative disorders such as Alzheimer's disease Hsp70 associates with amyloid-beta peptides potentially mitigating their aggregation toxicity. These interactions highlight Hsp70's importance in both protective and pathological cellular processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Heat shock 70 kDa protein 4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EXCLI journal 19:1141-1153 PubMed33013268

2020

LncRNA HOTAIR promotes MPP+-induced neuronal injury in Parkinson's disease by regulating the miR-874-5p/ATG10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingya Zhao,Hongli Li,Na Chang

View all publications

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Product promise

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