AB53496

Anti-Hsp70 antibody [BB70]

Name: Anti-Hsp70 antibody [BB70] (ab53496) | Abcam
Brand: Abcam Limited
SKU: AB53496
Price: 480 USD
Availability: InStock

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(10 Publications)

Mouse Monoclonal HSP70 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Cow, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

Heat shock 70 kDa protein, HSP70

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (AB53496)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (AB53496)

ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining.

Flow Cytometry - Anti-Hsp70 antibody [BB70] (AB53496)

Flow Cyt

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Flow Cytometry - Anti-Hsp70 antibody [BB70] (AB53496)

Overlay histogram showing Jurkat cells stained with ab53496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53496, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (AB53496)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (AB53496)

ab53496 staining Hsp70 in LLC-PK1 cells expressing Hsp70 under cold block conditions, by Immunocytochemistry/ Immunofluorescence.

Cells were fixed with 4% paraformaldehyde, permeabilized with 0.01% Triton X-100 in PBS for 4 minutes and blocked with 1% BSA/PBS for 20 minutes. Samples were incubated with primary antibody overnight at 4°C.

Image from Rice WL et al., PLoS One. 2012;7(2):e32843. Epub 2012 Feb 28. Fig 6.; doi:10.1371/journal.pone.0032843; February 28, 2012, PLoS ONE 7(2): e32843.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (AB53496)

ab53496, at a dilution of 1/200, staining Hsp70 + Hsc70 in liver smears of rat hepatocytes by Immunohistochemistry Formalin fixed Parafin embedded tissue.

1. Control

2. Two hours after exposure tp hyperthermic stress

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (AB53496)

ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in mouse colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (AB53496)

ab53496, at a 1/200 dilution, staining Hsp70 + Hsc70 in nuclear smears of rat hepatocytes by immunocytochemistry.

1. Control

2.Five hours after exposure to hyperthermic stress

Supplier Data

Western blot - Anti-Hsp70 antibody [BB70] (AB53496)

All lanes:

Western blot - Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 70 kDa

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Unknown

Western blot - Anti-Hsp70 antibody [BB70] (AB53496)

All lanes:

Western blot - Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution

All lanes:

Bovine MDBK cells

Predicted band size: 70 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BB70

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

Flow Cyt, IHC-P, WB, ICC/IF

applications

Specificity

Detects ~72 (Hsp) and ~73kDa (Hsc).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp70 also known as Heat Shock Protein 70 or HSPA1B is a molecular chaperone with a mass of approximately 70 kDa. It plays a mechanical role by assisting in the proper folding of nascent polypeptide chains and the refolding of misfolded proteins. Researchers often detect Hsp70 using Western blot and immunohistochemistry (IHC) techniques. Hsp70 is widely expressed in many tissues particularly during stress conditions like heat shock where its expression level increases significantly.

Biological function summary

Hsp70 operates by stabilizing intermediate states of folding proteins preventing aggregation and facilitating the correct folding process. It often forms a complex with co-chaperones such as Hsp40 and nucleotide exchange factors. This complex is essential for the protein's activity and function. Additionally Hsp70 participates in protein degradation pathways by guiding misfolded proteins to the proteasome for degradation maintaining cellular homeostasis.

Pathways

This molecular chaperone plays significant roles in the heat shock response and unfolded protein response pathways. Hsp70 interacts closely with proteins such as Hsp90 and co-chaperones which together help protect cells from stress-induced damage. The protein also participates in the JAK/STAT signaling pathway influencing cell proliferation and apoptosis. These interactions suggest an integral role in maintaining cellular integrity during stress conditions.

Overexpression of Hsp70 has been associated with various cancers and neurodegenerative diseases. In cancer Hsp70 helps tumor cells survive the hostile tumor microenvironment partly by interacting with anti-apoptotic proteins such as Bcl-2. In neurodegenerative disorders such as Alzheimer's disease Hsp70 associates with amyloid-beta peptides potentially mitigating their aggregation toxicity. These interactions highlight Hsp70's importance in both protective and pathological cellular processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.

See full target information Heat shock 70 kDa protein

Additional targets

Hsp70

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Pflugers Archiv : European journal of physiology 477:371-391 PubMed39836224

2025

Lateral hypothalamic area high-frequency deep brain stimulation rescues memory decline in aged rat: behavioral, molecular, and electrophysiological study.

Applications

Unspecified application

Species

Unspecified reactive species

Abdelaziz M Hussein,Ahmed F Abouelnaga,Walaa Obydah,Somaya Saad,Marwa Abass,Asmaa Yehia,Eman M Ibrahim,Ahmed T Ahmed,Osama A Abulseoud

Journal of animal science 101: PubMed37688555

2023

Heat shock protein 27 regulates myogenic and self-renewal potential of bovine satellite cells under heat stress.

Applications

Unspecified application

Species

Unspecified reactive species

Won Seob Kim,Jayasimha R Daddam,Boon Hong Keng,Jaehwan Kim,Jongkyoo Kim

Journal of cellular and molecular medicine 27:2165-2182 PubMed37386746

2023

HMSCs exosome-derived miR-199a-5p attenuates sulfur mustard-associated oxidative stress via the CAV1/NRF2 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chuchu Gong,Zhengyan Gu,Xinkang Zhang,Qingqiang Xu,Guanchao Mao,Zhipeng Pei,Wenqi Meng,Jinfeng Cen,Jihao Liu,Xiaowen He,Mingxue Sun,Kai Xiao

Frontiers in nutrition 9:877975 PubMed35571932

2022

Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature.

Applications

Unspecified application

Species

Unspecified reactive species

Changming Zhou,Xiaona Gao,Xianhong Cao,Guanming Tian,Cheng Huang,Lianying Guo,Yulan Zhao,Guoliang Hu,Ping Liu,Xiaoquan Guo

TheScientificWorldJournal 2021:8711286 PubMed34707467

2021

Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Roxb. Extract under Heat Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Sugito Sugito,Etriwati Etriwati,Muslim Akmal,Erdiansyah Rahmi,Mira Delima,Zainal Abidin Muchlisin,Denny Irmawati Hasan

International journal of molecular sciences 21: PubMed33255835

2020

Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A ().

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Wook Kim,Feriel Yasmine Mahiddine,Geon A Kim

Oxidative medicine and cellular longevity 2018:7014126 PubMed30425783

2018

Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Tang,Bin Yin,Jiao Xu,Endong Bao

The Journal of biological chemistry 293:3546-3561 PubMed29317494

2018

Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP).

Applications

Unspecified application

Species

Unspecified reactive species

Junghoon Ha,Yu Xu,Takeharu Kawano,Tyler Hendon,Lia Baki,Sumanta Garai,Andreas Papapetropoulos,Ganesh A Thakur,Leigh D Plant,Diomedes E Logothetis

International journal of molecular medicine 40:1201-1209 PubMed28849073

2017

Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Xiao,Yi Chai,Shigang Lv,Minhua Ye,Miaojing Wu,Liyuan Xie,Yanghua Fan,Xingen Zhu,Ziyun Gao

PloS one 7:e32843 PubMed22403603

2012

Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells.

Applications

ICC/IF

Species

Human

William L Rice,Yan Zhang,Ying Chen,Toshiyuki Matsuzaki,Dennis Brown,Hua A Jenny Lu

View all publications

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