Anti-Hsp90 alpha antibody [4B5]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [4B5] (AB314005)

Immunohistochemical analysis of ab314005 diluted at 1 : 80 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [4B5] (AB314005)

Immunohistochemical analysis of ab314005 diluted at 1 : 80 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• IP

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Immunoprecipitation - Anti-Hsp90 alpha antibody [4B5] (AB314005)

Immunoprecipitating Hsp90 in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 1 : Rabbit control IgG instead of ab314005 in HeLa whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2 : ab314005 (3μg) + HeLa whole cell lysate (500μg)

Lane 3 : HeLa whole cell lysate (20μg)

All lanes:

Immunoprecipitation - Anti-Hsp90 alpha antibody [4B5] (ab314005) at 3 µg

Predicted band size: 85 kDa

false

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [4B5] (AB314005)

Immunofluorescence staining of NIH/3T3 (Mouse embryonic fibroblast cell line) cells with ab314005 at 1 : 26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

• WB

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Western blot - Anti-Hsp90 alpha antibody [4B5] (AB314005)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp90 alpha antibody [4B5] (ab314005) at 0.8 µg/mL

Lane 1:

HeLa whole cell lysate

Lane 2:

MCF7 whole cell lysate

Lane 3:

K562 whole cell lysate

Lane 4:

HL-60 whole cell lysate

Lane 5:

HepG2 whole cell lysate

Lane 6:

A549 whole cell lysate

Lane 7:

Jurkat whole cell lysate

Lane 8:

PC3 whole cell lysate

Lane 9:

Rat brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 85 kDa

false