AB128483

Anti-Hsp90 alpha antibody [5G5]

Name: Anti-Hsp90 alpha antibody [5G5] (ab128483) | Abcam
Brand: Abcam Limited
SKU: AB128483
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal HS90A antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human, African green monkey, Rat, Mouse, Synthetic peptide, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSP90AA1.

View Alternative Names

HSP90A, HSPC1, HSPCA, HSP90AA1, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, Heat shock protein family C member 1, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38, HSP 86, HSP86, LAP-2, LPS-associated protein 2

6 Images

Flow Cytometry - Anti-Hsp90 alpha antibody [5G5] (AB128483)

Flow Cyt

Unknown

Flow Cytometry - Anti-Hsp90 alpha antibody [5G5] (AB128483)

ab128483, at a 1/200 dilution, staining Hsp90 alpha in HeLa cells by Flow Cytometry (green). Negative control is shown in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (AB128483)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (AB128483)

ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (AB128483)

ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.

ELISA - Anti-Hsp90 alpha antibody [5G5] (AB128483)

ELISA

Unknown

ELISA - Anti-Hsp90 alpha antibody [5G5] (AB128483)

ELISA using ab128483.

Unknown

Western blot - Anti-Hsp90 alpha antibody [5G5] (AB128483)

Expected MWt of recombinant protein is 50.5 kDa.

All lanes:

Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution

All lanes:

Human Hsp90 alpha (amino acids 275-484) recombinant protein

Predicted band size: 49 kDa,57 kDa,72 kDa,75 kDa,84 kDa,85 kDa

false

Unknown

Western blot - Anti-Hsp90 alpha antibody [5G5] (AB128483)

All lanes:

Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution

Lane 1:

NIH 3T3 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

HCT116 cell lysate

Lane 4:

HL-60 cell lysate

Lane 5:

SK-Br-3 cell lysate

Lane 6:

C6 cell lysate

Lane 7:

COS7 cell lysate

Predicted band size: 85 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G5

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

ELISA, Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HSP90AA1. The exact immunogen used to generate this antibody is proprietary information.

P07900

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp90 alpha also known as Hsp90AA1 or 5G5 is a molecular chaperone with an approximate molecular weight of 90 kDa. It plays an important role in the folding stability and function of many client proteins. This protein is expressed ubiquitously in eukaryotic cells with high levels in the cytoplasm and the endoplasmic reticulum. Hsp90 alpha operates through a unique ATPase-driven chaperone cycle that modulates protein conformations helping in the assembly and disassembly of protein complexes.

Biological function summary

Hsp90 alpha assists in maintaining protein homeostasis and is part of a larger chaperome complex including co-chaperones and other chaperones such as Hsp70. It plays an important role in stress response by stabilizing proteins and preventing aggregation. Hsp90 alpha is essential for the normal function of several kinases and transcription factors which are important for cell signaling and regulation processes. It functions as a mediator for cellular response to environmental cues.

Pathways

Hsp90 alpha is an integral component in the signal transduction and cell cycle control pathways. It interacts with various signaling proteins and receptors like AKT and steroid hormone receptors to facilitate their proper folding and activation. Furthermore Hsp90 alpha influences the MAPK/ERK pathway playing a role in cell proliferation and differentiation. Its interaction with Hsp90 protein clients creates a regulatory framework across multiple pathways allowing precise control over cellular function.

Hsp90 alpha is linked to cancer and neurodegenerative diseases. Its enhanced expression in tumors correlates with the stabilization of oncogenic client proteins such as HER2 contributing to cancer progression. Additionally Hsp90 alpha relates to disorders like Alzheimer's disease due to its role in handling misfolded proteins. The protein interacts with co-chaperones like H1L1 which may influence its impact on disease outcomes highlighting the therapeutic potential of targeting Hsp90 alpha in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed : 11274138, PubMed : 12526792, PubMed : 15577939, PubMed : 15937123, PubMed : 27353360, PubMed : 29127155). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself (PubMed : 29127155). Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed : 26991466, PubMed : 27295069). Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70 (PubMed : 12526792). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels (PubMed : 25973397). In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues (PubMed : 25973397). Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment (PubMed : 25973397). Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed : 25973397). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed : 11276205). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed : 24613385). Mediates the association of TOMM70 with IRF3 or TBK1 in mitochondrial outer membrane which promotes host antiviral response (PubMed : 20628368, PubMed : 25609812).. (Microbial infection) Seems to interfere with N.meningitidis NadA-mediated invasion of human cells. Decreasing HSP90 levels increases adhesion and entry of E.coli expressing NadA into human Chang cells; increasing its levels leads to decreased adhesion and invasion.

See full target information HSP90AA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 3:100820 PubMed36384103

2022

In vivo partial reprogramming by bacteria promotes adult liver organ growth without fibrosis and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Hess,Timothy J Kendall,Maria Pena,Keitaro Yamane,Daniel Soong,Linda Adams,Richard Truman,Anura Rambukkana

Oncology reports 44:2725-2734 PubMed33125132

2020

VDAC upregulation and αTAT1‑mediated α‑tubulin acetylation contribute to tanespimycin‑induced apoptosis in Calu‑1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qilin Wang,Xiangguo Liu

View all publications

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