Name: Anti-Hsp90 antibody [AC88]
SKU: ab13492
Rating: 4 (30 reviews)

Anti-Hsp90 antibody [AC88] (ab13492) is a mouse monoclonal antibody that is used to detect Hsp90 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Chinese hamster, Human, Mouse, Rat samples.

- Over 130 publications

- Trusted since 2004

Images

Western blot - Anti-Hsp90 antibody [AC88] (AB13492), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Form: Liquid
Clonality: Monoclonal

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Expected
Rat	Tested	Expected	Expected	Expected
Chinese hamster	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Chinese hamster	Dilution info -	Notes 84.7 (alpha) , 83.2 (beta)
Species Mouse	Dilution info -	Notes 84.7 (alpha) , 83.2 (beta)
Species Rat	Dilution info -	Notes 84.7 (alpha) , 83.2 (beta)
Species Human	Dilution info -	Notes 84.7 (alpha) , 83.2 (beta)

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Chinese hamster, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Chinese hamster, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Chinese hamster, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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6 products for Alternative Product

Target data

See full target information Hsp90

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Form: Liquid
Clonality: Monoclonal
Clone number: AC88
Purification technique: Affinity purification Protein G
Epitope: The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Hsp90 antibody [AC88] (ab13492) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Chinese hamster, Human, Mouse, Rat samples.

Trusted by the scientific community

Anti-Hsp90 [AC88] (ab13492) was first used in a scientific publication in 2004 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists

Anti-Hsp90 [AC88] (ab13492) has over 30 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp90 also known as heat shock protein 90 is a molecular chaperone with a mass of about 90 kDa. It assists in the proper folding of client proteins stabilization of proteins against heat stress and degradation of misfolded proteins. Hsp90 is present in various cellular compartments including the cytoplasm nucleus and mitochondria. It is highly expressed in most eukaryotic cells reflecting its fundamental role in maintaining cellular protein homeostasis. Additionally Hsp90 serves as a loading control in western blot experiments due to its consistent expression levels across samples.

Biological function summary

Hsp90 interacts with many co-chaperones to form multi-protein complexes that aid its function. This protein is necessary for the maturation and stability of many signaling proteins including steroid hormone receptors and kinases like the tyrosine kinase D7A. Hsp90's chaperone activity is ATP-dependent with its N-terminal domain binding and hydrolyzing ATP leading to conformational changes that promote protein folding and assembly. Its influence extends to regulating cell cycle control and apoptosis highlighting its importance in cellular processes.

Pathways

Hsp90 participates in key biological pathways such as the protein folding response and the MAPK signaling pathway. In the protein folding process Hsp90 collaborates with co-chaperones like Aha1 and p23 to ensure accurate protein synthesis and repair. Its role in the MAPK signaling pathway influences cell growth proliferation and differentiation interacting with proteins like Raf-1 and MEK. These interactions highlight Hsp90's involvement in signal transduction and cellular stress responses.

Associated diseases and disorders

Hsp90 is implicated in cancer and neurodegenerative diseases. Its overexpression often correlates with tumor progression and poor prognosis in cancers where it stabilizes client proteins like HER2 and AKT that drive oncogenic processes. In neurodegenerative disorders such as Alzheimer's disease altered Hsp90 function affects the degradation of proteins like tau contributing to pathogenic protein aggregation. Understanding Hsp90's role in these conditions offers avenues for therapeutic interventions targeting its chaperone activity.

Product promise

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8 product images

Western blot - Anti-Hsp90 antibody [AC88] (ab13492)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution
Lane 1: Hsp90 native human protein
Lane 2: Hsp90 beta reombinant human protein
Lane 3: Hsp90 alpha reombinant human protein
Lane 4: Cell lysates prepared from heat shocked Hela cells
Lane 5: Cell lysates prepared from heat shocked 3T3 cells
Lane 6: Cell lysates prepared from heat shocked PC-12 cells
Lane 7: Cell lysates prepared from heat shocked CHO-K1 cells
Lane 8: Cell lysates prepared from heat shocked Rat-2 cells
Predicted band size: 85 kDa
This image is courtesy of a customer review submitted by Armen Petrosyan
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [AC88] (ab13492)
ab13492 staining Hsp90 (red) and another antibody to C2GnT-M (Golgi enzyme, greeen) in Panc-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
This image was generated using the ascites version of the product.
Western blot - Anti-Hsp90 antibody [AC88] (ab13492)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution
Lane 2: HSP90 cell lysate
Lane 3: HSP90β cell lysate
Lane 4: HSP90α cell lysate
Lane 5: HeLa (heat shocked) cell lysate
Lane 6: NIH/3T3 (Heat shocked) cell lysate
Lanes 7 - 8: PC-12 (Heat shocked) cell lysate
Predicted band size: 85 kDa
This image is courtesy of an anonymous customer review
Western blot - Anti-Hsp90 antibody [AC88] (ab13492)
Blocked with 5% Milk for 2 hours at 22°C
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution
All lanes: Human fibroblast whole cell lysate at 40 µg
Secondary
All lanes: HRP-conjugated goat anti-mouse polyclonal IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 20s
This image is courtesy of a customer review submitted by Rudolf Jung
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [AC88] (ab13492)
ab13492 staining Hsp90 in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/500) for 30 minutes at 20°C. A HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody.
This image was generated using the ascites version of the product.
Flow Cytometry (Intracellular) - Anti-Hsp90 antibody [AC88] (ab13492)
Overlay histogram showing HeLa cells stained with ab13492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (Normal Goat Serum ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13492, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [AC88] (ab13492)
ab13492 staining Hsp90 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (10 ug/ml).
This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody [AC88] (ab13492)
ICC/IF image of ab13492 stained HepG2 cells (ab7900). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13492, 5μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight^® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.