AB32568

Anti-Hsp90 beta antibody [E296]

20ul selling size
RabMAb
Recombinant
KO Validated
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(20 Publications)

Rabbit Recombinant Monoclonal HS90B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 20 publications.

View Alternative Names

HSP90B, HSPC2, HSPC3, HSPCB, HSP90AB1, Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, Heat shock protein family C member 3, HSP 84, HSP84

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (AB32568)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (AB32568)

Immunofluorescence staining of HepG2 cells with purified ab32568 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 555 goat anti rabbit (ab150082), used at a dilution of 1 in 400. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab32568 was used at a dilution of 1/200 followed by an Alexa Fluor^® 488 goat anti-mouse antibody at a dilution of 1/500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568)

Immunohistochemical staining of paraffin embedded human stomach with purified ab32568 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568)

Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using unpurified ab32568 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Lanes 1 - 4 : Merged signal (red and green). Green - ab32568 observed at 85 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab32568 was shown to react with Hsp90 beta in wild-type HEK-293T cells in western blot with loss of signal observed in HSP90AB1 knockout cell line ab266117 (HSP90AB1 knockout cell lysate ab257190). Wild-type and HSP90AB1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab32568 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 200000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/200000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

HSP90AB1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hsp90ab1-hsp90-beta-knockout-hek-293t-cell-line-ab266117'>ab266117</a>)

Lane 3:

Saos-2 cell lysate at 20 µg

Lane 4:

HL-60 cell lysate at 20 µg

Predicted band size: 83 kDa

Observed band size: 85 kDa

false

Lab

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 83 kDa

Observed band size: 90 kDa

false

Unknown

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

All lanes:

Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/500 dilution

All lanes:

Hela cell lysate

Predicted band size: 83 kDa

Observed band size: 92 kDa

false

Lab

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Lanes 1-4 : Merged signal (red and green). Green - ab32568 observed at 90 kDa. Red - loading control ab8245 observed at 36 kDa.

ab32568 Anti-Hsp90 beta antibody [E296] was shown to specifically react with Hsp90 beta in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266117 (knockout cell lysate ab257190) was used. Wild-type and Hsp90 beta knockout samples were subjected to SDS-PAGE. ab32568 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HSP90AB1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hsp90ab1-hsp90-beta-knockout-hek-293t-cell-line-ab266117'>ab266117</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 83 kDa

Observed band size: 90 kDa

false

Lab

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 83 kDa

Observed band size: 90 kDa

false

Lab

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

All lanes:

Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution

All lanes:

SH-SH5Y cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 83 kDa

Observed band size: 90 kDa

false

Carrier free

Anti-Hsp90 beta antibody [E296] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E296

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000 - 1/500000", "WB-species-notes": "For unpurified, use 1/500.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000 - 1/500000", "WB-species-notes": "For unpurified, use 1/500.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000 - 1/500000", "WB-species-notes": "For unpurified, use 1/500.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp90 beta also known as Hsp90AB1 or Hsp90 protein is a heat shock protein of approximately 90 kDa. It is a molecular chaperone found in most eukaryotic cells. Hsp90 beta helps in the proper folding stabilization and degradation of many proteins. Unlike its isoform Hsp90 alpha Hsp90 beta has a more stable expression and is not typically induced by stress. This protein is primarily localized in the cytosol but can also be present in other cellular compartments depending on the cellular state.

Biological function summary

Hsp90 beta functions to maintain protein homeostasis and cellular integrity. It forms part of a multi-protein chaperone complex which includes cochaperones such as Hop Hsp70 and p23 necessary for its full functionality. Hsp90 beta supports the maturation of steroid hormone receptors kinases and other client proteins. It plays an important role in the cell cycle regulation through its interaction with various proteins ensuring proper cell division and growth.

Pathways

Hsp90 beta is deeply involved in signal transduction and cellular stress response pathways. Its interaction with the Akt pathway is significant for cell survival signals. Hsp90 beta also participates in the MAP kinase pathway affecting cell growth and differentiation. The protein associates with multiple kinases including RAF and Src which are important for downstream signaling.

The dysregulation of Hsp90 beta is associated with cancer and neurodegenerative diseases. In cancer Hsp90 beta stabilizes many oncoproteins making it a potential therapeutic target for inhibiting tumor growth. It interacts with client proteins like the proto-oncogene c-Src promoting tumorigenesis. In neurodegenerative disorders such as Alzheimer's disease improper interaction between Hsp90 beta and tau proteins can contribute to disease progression impacting neuronal function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed : 16478993, PubMed : 19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed : 26991466, PubMed : 27295069). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed : 25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed : 24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed : 18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed : 20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed : 32272059).. (Microbial infection) Binding to N.meningitidis NadA stimulates monocytes (PubMed : 21949862). Seems to interfere with N.meningitidis NadA-mediated invasion of human cells (Probable).

See full target information HSP90AB1

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:643 PubMed37773169

2023

Cerebral endothelial cell-derived extracellular vesicles regulate microglial polarization and promote autophagy via delivery of miR-672-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Changshui Wang,Lei Feng,Li Zhu,Linlin Wu,Beibei Chen,Changmeng Cui,Mengqi Yang,Yahao Gao,Pei Jiang

Nature communications 14:5178 PubMed37620331

2023

Biosynthesized gold nanoparticles that activate Toll-like receptors and elicit localized light-converting hyperthermia for pleiotropic tumor immunoregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Qin,Yang Chen,Zeming Wang,Nan Li,Qing Sun,Yixuan Lin,Wenyi Qiu,Yuting Qin,Long Chen,Hanqing Chen,Yiye Li,Jian Shi,Guangjun Nie,Ruifang Zhao

International journal of molecular sciences 24: PubMed36982769

2023

Valproic Acid Inhibits Progressive Hereditary Hearing Loss in a KCNQ4 Variant Model through HDAC1 Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Seok Nam,Young Mi Choi,Sungsu Lee,Hyong-Ho Cho

Stem cells international 2022:2760899 PubMed36199626

2022

E3 Ubiquitin Ligase CHIP Inhibits the Interaction between Hsp90 and MAST1 to Repress Radiation Resistance in Non-Small-Cell Lung Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Tan,Jingwei Zhang,Wen Wang,Haibo Ma,Yuanyuan Yang

Bioactive materials 12:185-197 PubMed35310379

2022

Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Pengzhen Cheng,Tianqing Cao,Xueyi Zhao,Weiguang Lu,Sheng Miao,Fenru Ning,Dong Wang,Yi Gao,Long Wang,Guoxian Pei,Liu Yang

Cell death discovery 8:86 PubMed35220409

2022

NVP-AUY922 alleviates radiation-induced lung injury via inhibition of autophagy-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Dongming Wu,Shihua Deng,Jin Li,Feng Zhang,Ye Zou,Ting Zhang,Ying Xu

Cell reports. Medicine 3:100502 PubMed35243415

2022

SU086, an inhibitor of HSP90, impairs glycolysis and represents a treatment strategy for advanced prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan A Rice,Vineet Kumar,Dhanir Tailor,Fernando Jose Garcia-Marques,En-Chi Hsu,Shiqin Liu,Abel Bermudez,Vijayalakshmi Kanchustambham,Vishnu Shankar,Zintis Inde,Busola Ruth Alabi,Arvind Muruganantham,Michelle Shen,Mallesh Pandrala,Rosalie Nolley,Merve Aslan,Ali Ghoochani,Arushi Agarwal,Mark Buckup,Manoj Kumar,Catherine C Going,Donna M Peehl,Scott J Dixon,Richard N Zare,James D Brooks,Sharon J Pitteri,Sanjay V Malhotra,Tanya Stoyanova

Scientific reports 11:22925 PubMed34824343

2021

Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Jun Shih,Hsin-Fang Chang,Chi-Ling Chen,Pao-Ling Torng

Macromolecular bioscience 22:e2100353 PubMed34762334

2021

Dual Photothermal/Chemotherapy of Melanoma Cells with Albumin Nanoparticles Carrying Indocyanine Green and Doxorubicin Leads to Immunogenic Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Niloofar Heshmati Aghda,Susana Torres Hurtado,Shahad M Abdulsahib,Emilio J Lara,James W Tunnell,Tania Betancourt

Cell stem cell 28:1549-1565.e12 PubMed33915080

2021

Principles of signaling pathway modulation for enhancing human naive pluripotency induction.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Bayerl,Muneef Ayyash,Tom Shani,Yair Shlomo Manor,Ohad Gafni,Rada Massarwa,Yael Kalma,Alejandro Aguilera-Castrejon,Mirie Zerbib,Hadar Amir,Daoud Sheban,Shay Geula,Nofar Mor,Leehee Weinberger,Segev Naveh Tassa,Vladislav Krupalnik,Bernardo Oldak,Nir Livnat,Shadi Tarazi,Shadi Tawil,Emilie Wildschutz,Shahd Ashouokhi,Lior Lasman,Varda Rotter,Suhair Hanna,Dalit Ben-Yosef,Noa Novershtern,Sergey Viukov,Jacob H Hanna

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hsp90 beta antibody [E296]

Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (AB32568)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568)

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise