Rabbit Recombinant Monoclonal HS90B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 17 publications.
Images
![Western blot - Anti-Hsp90 beta antibody [E296] (AB32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img2873.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34640.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (AB32568)")
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (AB32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--immunocytochemistry-immunofluorescence-img14735.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (AB32568)")
![Western blot - Anti-Hsp90 beta antibody [E296] (AB32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img287.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)")
![Western blot - Anti-Hsp90 beta antibody [E296] (AB32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img110401.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [E296] (AB32568)")
Publications
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- Nature communications 14:51782023Biosynthesized gold nanoparticles that activate Toll-like receptors and elicit localized light-converting hyperthermia for pleiotropic tumor immunoregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Qin,Yang Chen,Zeming Wang,Nan Li,Qing Sun,Yixuan Lin,Wenyi Qiu,Yuting Qin,Long Chen,Hanqing Chen,Yiye Li,Jian Shi,Guangjun Nie,Ruifang ZhaoPubMed 37620331
- Bioactive materials 12:185-1972022Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengzhen Cheng,Tianqing Cao,Xueyi Zhao,Weiguang Lu,Sheng Miao,Fenru Ning,Dong Wang,Yi Gao,Long Wang,Guoxian Pei,Liu YangPubMed 35310379
- Cell death discovery 8:862022NVP-AUY922 alleviates radiation-induced lung injury via inhibition of autophagy-dependent ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Dongming Wu,Shihua Deng,Jin Li,Feng Zhang,Ye Zou,Ting Zhang,Ying XuPubMed 35220409
- Cell reports. Medicine 3:1005022022SU086, an inhibitor of HSP90, impairs glycolysis and represents a treatment strategy for advanced prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeghan A Rice,Vineet Kumar,Dhanir Tailor,Fernando Jose Garcia-Marques,En-Chi Hsu,Shiqin Liu,Abel Bermudez,Vijayalakshmi Kanchustambham,Vishnu Shankar,Zintis Inde,Busola Ruth Alabi,Arvind Muruganantham,Michelle Shen,Mallesh Pandrala,Rosalie Nolley,Merve Aslan,Ali Ghoochani,Arushi Agarwal,Mark Buckup,Manoj Kumar,Catherine C Going,Donna M Peehl,Scott J Dixon,Richard N Zare,James D Brooks,Sharon J Pitteri,Sanjay V Malhotra,Tanya StoyanovaPubMed 35243415
- Scientific reports 11:229252021Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHo-Jun Shih,Hsin-Fang Chang,Chi-Ling Chen,Pao-Ling TorngPubMed 34824343
- Macromolecular bioscience 22:e21003532021Dual Photothermal/Chemotherapy of Melanoma Cells with Albumin Nanoparticles Carrying Indocyanine Green and Doxorubicin Leads to Immunogenic Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNiloofar Heshmati Aghda,Susana Torres Hurtado,Shahad M Abdulsahib,Emilio J Lara,James W Tunnell,Tania BetancourtPubMed 34762334
- Cell stem cell 28:1549-1565.e122021Principles of signaling pathway modulation for enhancing human naive pluripotency induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Bayerl,Muneef Ayyash,Tom Shani,Yair Shlomo Manor,Ohad Gafni,Rada Massarwa,Yael Kalma,Alejandro Aguilera-Castrejon,Mirie Zerbib,Hadar Amir,Daoud Sheban,Shay Geula,Nofar Mor,Leehee Weinberger,Segev Naveh Tassa,Vladislav Krupalnik,Bernardo Oldak,Nir Livnat,Shadi Tarazi,Shadi Tawil,Emilie Wildschutz,Shahd Ashouokhi,Lior Lasman,Varda Rotter,Suhair Hanna,Dalit Ben-Yosef,Noa Novershtern,Sergey Viukov,Jacob H HannaPubMed 33915080
- Breast cancer research and treatment 184:237-2482020Effect of functional variant rs11466313 on breast cancer susceptibility and TGFB1 promoter activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Ting Zhou et. al.PubMed 32757134
- BMC cardiovascular disorders 19:3062019Heat shock protein 90 is downregulated in calcific aortic valve disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonna Weisell et. al.PubMed 31856737
- Frontiers in endocrinology 10:4872019Heat Shock Protein 90 as a Prognostic Marker and Therapeutic Target for Adrenocortical Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Siebert et. al.PubMed 31379752
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Expected | Not recommended | Tested | Expected |
| Rat | Expected | Not recommended | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100000 - 1/500000 | Notes For unpurified, use 1/500. |
Species Human | Dilution info 1/100000 - 1/500000 | Notes For unpurified, use 1/500. |
Species Rat | Dilution info 1/100000 - 1/500000 | Notes For unpurified, use 1/500. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:26991466, PubMed:27295069). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059). (Microbial infection) Binding to N.meningitidis NadA stimulates monocytes (PubMed:21949862). Seems to interfere with N.meningitidis NadA-mediated invasion of human cells (Probable).
Alternative names
HSP90B, HSPC2, HSPC3, HSPCB, HSP90AB1, Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, Heat shock protein family C member 3, HSP 84, HSP84
Recommended products
Rabbit Recombinant Monoclonal HS90B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 17 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E296
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp90 beta also known as Hsp90AB1 or Hsp90 protein is a heat shock protein of approximately 90 kDa. It is a molecular chaperone found in most eukaryotic cells. Hsp90 beta helps in the proper folding stabilization and degradation of many proteins. Unlike its isoform Hsp90 alpha Hsp90 beta has a more stable expression and is not typically induced by stress. This protein is primarily localized in the cytosol but can also be present in other cellular compartments depending on the cellular state.
- Biological function summary
Hsp90 beta functions to maintain protein homeostasis and cellular integrity. It forms part of a multi-protein chaperone complex which includes cochaperones such as Hop Hsp70 and p23 necessary for its full functionality. Hsp90 beta supports the maturation of steroid hormone receptors kinases and other client proteins. It plays an important role in the cell cycle regulation through its interaction with various proteins ensuring proper cell division and growth.
- Pathways
Hsp90 beta is deeply involved in signal transduction and cellular stress response pathways. Its interaction with the Akt pathway is significant for cell survival signals. Hsp90 beta also participates in the MAP kinase pathway affecting cell growth and differentiation. The protein associates with multiple kinases including RAF and Src which are important for downstream signaling.
- Associated diseases and disorders
The dysregulation of Hsp90 beta is associated with cancer and neurodegenerative diseases. In cancer Hsp90 beta stabilizes many oncoproteins making it a potential therapeutic target for inhibiting tumor growth. It interacts with client proteins like the proto-oncogene c-Src promoting tumorigenesis. In neurodegenerative disorders such as Alzheimer's disease improper interaction between Hsp90 beta and tau proteins can contribute to disease progression impacting neuronal function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img2873.jpg "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)
Lanes 1 - 4: Merged signal (red and green). Green - ab32568 observed at 85 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.ab32568 was shown to react with Hsp90 beta in wild-type HEK-293T cells in western blot with loss of signal observed in HSP90AB1 knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (HSP90AB1 knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190). Wild-type and HSP90AB1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab32568 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 200000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/200000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HSP90AB1 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line (Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117)
Lane 3: Saos-2 cell lysate at 20 µg
Lane 4: HL-60 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34640.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568)
Immunohistochemical staining of paraffin embedded human stomach with purified ab32568 at a working dilution of 1 in 150. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--immunocytochemistry-immunofluorescence-img14735.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (ab32568)")
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [E296] (ab32568)
Immunofluorescence staining of HepG2 cells with purified ab32568 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed ab150082), used at a dilution of 1 in 400. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab32568 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img287.jpg "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)
Lanes 1-4: Merged signal (red and green). Green - ab32568 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab32568 Anti-Hsp90 beta antibody [E296] was shown to specifically react with Hsp90 beta in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190) was used. Wild-type and Hsp90 beta knockout samples were subjected to SDS-PAGE. ab32568 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: HSP90AB1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line (Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117)
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img110401.jpg "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img81889.jpg "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)
All lanes: Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution
All lanes: SH-SH5Y cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img99580.jpg "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/100000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
Lane 3: Raji cell lysate at 20 µg
Lane 4: A431 cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img89217.gif "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)
All lanes: Western blot - Anti-Hsp90 beta antibody [E296] (ab32568) at 1/500 dilution
All lanes: Hela cell lysate
Predicted band size: 83 kDa
Observed band size: 92 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114963.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568)
Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using unpurified ab32568 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img81889.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
![Western blot - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--western-blot-img99580.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [E296] (ab32568)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-e296-ab32568--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114963.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [E296] (ab32568)")
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