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Rabbit Recombinant Monoclonal HS90B antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment, Human, Mouse, Rat samples. Cited in 13 publications.

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Images

Western blot - Anti-Hsp90 beta antibody [EPR16621] (AB203085), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] (AB203085), expandable thumbnail
  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (AB203085), expandable thumbnail
  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (AB203085), expandable thumbnail
  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (AB203085), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested
Recombinant fragment
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Recombinant fragment

Dilution info

1/70

Notes

-

Species

Human

Dilution info

1/70

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Recombinant fragment

Dilution info

1/5000

Notes

-

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/5000

Notes

-

Species

Human

Dilution info

1/5000

Notes

-

Tested
Tested

Species

Recombinant fragment

Dilution info

1/250

Notes

-

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Recombinant fragment

Dilution info

1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Recombinant fragment

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).

Alternative names

Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84, HSPCB, HSPC2, HSP90B, HSP90AB1

Recommended products

Rabbit Recombinant Monoclonal HS90B antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment, Human, Mouse, Rat samples. Cited in 13 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16621

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp90 beta also known as Hsp90AB1 or Hsp90 protein is a heat shock protein of approximately 90 kDa. It is a molecular chaperone found in most eukaryotic cells. Hsp90 beta helps in the proper folding stabilization and degradation of many proteins. Unlike its isoform Hsp90 alpha Hsp90 beta has a more stable expression and is not typically induced by stress. This protein is primarily localized in the cytosol but can also be present in other cellular compartments depending on the cellular state.

Biological function summary

Hsp90 beta functions to maintain protein homeostasis and cellular integrity. It forms part of a multi-protein chaperone complex which includes cochaperones such as Hop Hsp70 and p23 necessary for its full functionality. Hsp90 beta supports the maturation of steroid hormone receptors kinases and other client proteins. It plays an important role in the cell cycle regulation through its interaction with various proteins ensuring proper cell division and growth.

Pathways

Hsp90 beta is deeply involved in signal transduction and cellular stress response pathways. Its interaction with the Akt pathway is significant for cell survival signals. Hsp90 beta also participates in the MAP kinase pathway affecting cell growth and differentiation. The protein associates with multiple kinases including RAF and Src which are important for downstream signaling.

Associated diseases and disorders

The dysregulation of Hsp90 beta is associated with cancer and neurodegenerative diseases. In cancer Hsp90 beta stabilizes many oncoproteins making it a potential therapeutic target for inhibiting tumor growth. It interacts with client proteins like the proto-oncogene c-Src promoting tumorigenesis. In neurodegenerative disorders such as Alzheimer's disease improper interaction between Hsp90 beta and tau proteins can contribute to disease progression impacting neuronal function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Lanes 1 - 4: Merged signal (red and green). Green - ab203085 observed at 85 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab203085 was shown to react with Hsp90 beta in wild-type HEK-293T cells in western blot with loss of signal observed in HSP90AB1 knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (HSP90AB1 knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190). Wild-type and HSP90AB1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab203085 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/5000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: HSP90AB1 knockout HEK-293T cell lysate at 20 µg

    Lane 3: Saos-2 cell lysate at 20 µg

    Lane 4: HL-60 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 85 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling Hsp90 beta with ab203085 at a 1/250 dilution showing cytoplasmic staining. Permeabilisation using 0.1% tritonX-100.

    Secondary ab: Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178854 at 1/250 dilution, Anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/400 dilution. -ve control 2 is Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution, Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution.

  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Blocking and Diluting buffer and concentration: 5%
    NFDM/TBST.

    ab203085 only recognizes Hsp90 beta based on recombinant protein WB result.

    All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/10000 dilution

    Lane 1: Hsp90 alpha recombinant protein fragment (GST-tag): aa533-732 at 0.01 µg

    Lane 2: Hsp90 beta recombinant protein fragment (His-Tag®): aa525-724 at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 83 kDa

    Exposure time: 3min

  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Lanes 1-4: Merged signal (red and green). Green - ab203085 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab203085 Anti-Hsp90 beta antibody [EPR16621] was shown to specifically react with Hsp90 beta in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190) was used. Wild-type and Hsp90 beta knockout samples were subjected to SDS-PAGE. ab203085 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: HSP90AB1 knockout HEK293T cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: SH-SY5Y cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 83 kDa

    Observed band size: 90 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab203085).

    Lanes 1-4: Merged signal (red and green). Green - ab203085 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab203085 Anti-Hsp90 beta antibody [EPR16621] was shown to specifically react with Hsp90 beta in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190) was used. Wild-type and Hsp90 beta knockout samples were subjected to SDS-PAGE. ab203085 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/20000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Lane 2: 293T (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg

    Lane 3: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

    Observed band size: 90 kDa

  • Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/5000 dilution

    Lane 1: Mouse brain at 10 µg

    Lane 2: Mouse heart at 10 µg

    Lane 3: Mouse kidney at 10 µg

    Lane 4: Mouse spleen at 10 µg

    Lane 5: Rat brain at 10 µg

    Lane 6: Rat heart at 10 µg

    Lane 7: Rat kidney at 10 µg

    Lane 8: Rat spleen at 10 µg

    Lane 9: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa

    Observed band size: 90 kDa

  • Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Hsp90 beta was immunoprecipitated from 1mg of Hela (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab203085 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab203085 at 1/5000 dilution. Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated, was used as secondary antibody at 1:1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Predicted band size: 83 kDa

    Observed band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depend on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depending on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depending on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] (ab203085), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] (ab203085)

    Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixedHeLa (Human epithelial cells from cervix adenocarcinoma) cells using ab203085 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (negative) (black). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

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