Rabbit Recombinant Monoclonal HS90B antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant fragment, Mouse, Rat, Human samples.
Images
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img101050.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390)")
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunocytochemistry-immunofluorescence-img13054.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390)")
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img148455.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390)")
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img160664.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390)")
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img34994.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (AB251390)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected | Expected |
| Mouse | Expected | Predicted | Expected | Expected | Expected |
| Rat | Expected | Predicted | Expected | Expected | Expected |
| Recombinant fragment | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:26991466, PubMed:27295069). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059). (Microbial infection) Binding to N.meningitidis NadA stimulates monocytes (PubMed:21949862). Seems to interfere with N.meningitidis NadA-mediated invasion of human cells (Probable).
Alternative names
HSP90B, HSPC2, HSPC3, HSPCB, HSP90AB1, Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, Heat shock protein family C member 3, HSP 84, HSP84
Recommended products
Rabbit Recombinant Monoclonal HS90B antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant fragment, Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16621
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251390 is the carrier-free version of Anti-Hsp90 beta antibody [EPR16621] ab203085.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hsp90 beta also known as Hsp90AB1 or Hsp90 protein is a heat shock protein of approximately 90 kDa. It is a molecular chaperone found in most eukaryotic cells. Hsp90 beta helps in the proper folding stabilization and degradation of many proteins. Unlike its isoform Hsp90 alpha Hsp90 beta has a more stable expression and is not typically induced by stress. This protein is primarily localized in the cytosol but can also be present in other cellular compartments depending on the cellular state.
- Biological function summary
Hsp90 beta functions to maintain protein homeostasis and cellular integrity. It forms part of a multi-protein chaperone complex which includes cochaperones such as Hop Hsp70 and p23 necessary for its full functionality. Hsp90 beta supports the maturation of steroid hormone receptors kinases and other client proteins. It plays an important role in the cell cycle regulation through its interaction with various proteins ensuring proper cell division and growth.
- Pathways
Hsp90 beta is deeply involved in signal transduction and cellular stress response pathways. Its interaction with the Akt pathway is significant for cell survival signals. Hsp90 beta also participates in the MAP kinase pathway affecting cell growth and differentiation. The protein associates with multiple kinases including RAF and Src which are important for downstream signaling.
- Associated diseases and disorders
The dysregulation of Hsp90 beta is associated with cancer and neurodegenerative diseases. In cancer Hsp90 beta stabilizes many oncoproteins making it a potential therapeutic target for inhibiting tumor growth. It interacts with client proteins like the proto-oncogene c-Src promoting tumorigenesis. In neurodegenerative disorders such as Alzheimer's disease improper interaction between Hsp90 beta and tau proteins can contribute to disease progression impacting neuronal function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img101050.jpg "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Western blot staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Hsp90 beta antibody [EPR16621] ab203085 observed at 85 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-Hsp90 beta antibody [EPR16621] ab203085 was shown to react with Hsp90 beta in wild-type HEK-293T cells in western blot with loss of signal observed in HSP90AB1 knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (HSP90AB1 knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190). Wild-type and HSP90AB1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-Hsp90 beta antibody [EPR16621] ab203085 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (Anti-Hsp90 beta antibody [EPR16621] ab203085) at 1/5000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line (Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117)
Lane 2: HSP90AB1 knockout HEK-293T cell lysate at 20 µg
Lane 3: Saos-2 cell lysate at 20 µg
Lane 4: HL-60 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunocytochemistry-immunofluorescence-img13054.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling Hsp90 beta with Anti-Hsp90 beta antibody [EPR16621] ab203085 at a 1/250 dilution showing cytoplasmic staining. Permeabilisation using 0.1% tritonX-100.
Secondary ab: Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178854 at 1/250 dilution, Anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/400 dilution. -ve control 2 is Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/500 dilution, Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution.
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img148455.jpg "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Western blot staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Lanes 1-4: Merged signal (red and green). Green - Anti-Hsp90 beta antibody [EPR16621] ab203085 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-Hsp90 beta antibody [EPR16621] ab203085 Anti-Hsp90 beta antibody [EPR16621] was shown to specifically react with Hsp90 beta in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190) was used. Wild-type and Hsp90 beta knockout samples were subjected to SDS-PAGE. Anti-Hsp90 beta antibody [EPR16621] ab203085 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (Anti-Hsp90 beta antibody [EPR16621] ab203085) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line (Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117)
Lane 2: HSP90AB1 knockout HEK293T cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img160664.jpg "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Western blot staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (Anti-Hsp90 beta antibody [EPR16621] ab203085) at 1/20000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 2: 293T (Human epithelial cells from embryonic kidney) whole cell lysates at 20 µg
Lane 3: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg
Lane 4: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--western-blot-img34994.jpg "Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Western blot - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Western blot staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-Hsp90 beta antibody [EPR16621] (Anti-Hsp90 beta antibody [EPR16621] ab203085) at 1/5000 dilution
Lane 1: Mouse brain at 10 µg
Lane 2: Mouse heart at 10 µg
Lane 3: Mouse kidney at 10 µg
Lane 4: Mouse spleen at 10 µg
Lane 5: Rat brain at 10 µg
Lane 6: Rat heart at 10 µg
Lane 7: Rat kidney at 10 µg
Lane 8: Rat spleen at 10 µg
Lane 9: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunoprecipitation-img155708.jpg "Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Hsp90 beta was immunoprecipitated from 1mg of Hela (Human epithelial cells from cervix adenocarcinoma) whole cell extract with Anti-Hsp90 beta antibody [EPR16621] ab203085 at 1/70 dilution. Western blot was performed of the immunoprecipitate using Anti-Hsp90 beta antibody [EPR16621] ab203085 at 1/5000 dilution. Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated, was used as secondary antibody at 1:1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] (Anti-Hsp90 beta antibody [EPR16621] ab203085)
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88458.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Hsp90 beta with Anti-Hsp90 beta antibody [EPR16621] ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depend on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131342.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Hsp90 beta with Anti-Hsp90 beta antibody [EPR16621] ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depending on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106418.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Hsp90 beta with Anti-Hsp90 beta antibody [EPR16621] ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depending on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--flow-cytometry-intracellular-img37298.jpg "Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)
Hsp90 beta Flow Cytometry (Intracellular) staining using rabbit Anti-Hsp90 beta antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hsp90 beta antibody [EPR16621] ab203085).
Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixedHeLa (Human epithelial cells from cervix adenocarcinoma) cells using Anti-Hsp90 beta antibody [EPR16621] ab203085 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (negative) (black). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
![Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunoprecipitation-img155708.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88458.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131342.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106418.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
![Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390), expandable thumbnail](https://content.abcam.com/products/images/hsp90-beta-antibody-epr16621-bsa-and-azide-free-ab251390--flow-cytometry-intracellular-img37298.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [EPR16621] - BSA and Azide free (ab251390)")
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