Skip to main content

Mouse Monoclonal HS90B antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSP90AB1.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (AB53497), expandable thumbnail
  • Western blot - Anti-Hsp90 beta antibody [H90-10] (AB53497), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (AB53497), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [H90-10] (AB53497), expandable thumbnail
  • Western blot - Anti-Hsp90 beta antibody [H90-10] (AB53497), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human HSP90AB1. Database link P08238

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

0.5 µg for 106 Cells

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:26991466, PubMed:27295069). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).(Microbial infection) Binding to N.meningitidis NadA stimulates monocytes (PubMed:21949862). Seems to interfere with N.meningitidis NadA-mediated invasion of human cells (Probable).

Alternative names

HSP90B, HSPC2, HSPC3, HSPCB, HSPCB, HSPC2, HSP90B, HSP90AB1, Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, Heat shock protein family C member 3, HSP 84, HSP84

Recommended products

Mouse Monoclonal HS90B antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSP90AB1.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human HSP90AB1. Database link P08238
Clone number

H90-10

Purification technique

Affinity purification Protein G

Specificity

Detects 90kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    ab53497 staining Human normal placenta. Staining is localized to cytoplasmic compartment.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Lanes 1 - 4: Merged signal (red and green). Green - ab53497 observed at 85 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

    ab53497 was shown to react with Hsp90 beta in wild-type HEK-293T cells in western blot with loss of signal observed in HSP90AB1 knockout cell line Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell line ab266117 (HSP90AB1 knockout cell lysate Human HSP90AB1 (Hsp90 beta) knockout HEK-293T cell lysate ab257190). Wild-type and HSP90AB1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab53497 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497) at 1/5000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: HSP90AB1 knockout HEK-293T cell lysate at 20 µg

    Lane 3: Saos-2 cell lysate at 20 µg

    Lane 4: HL-60 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Immunohistochemistry analysis of formalin-fixed paraffin-embedded inflamed mouse colon using ab53497 at 1/10000. Primary antibody was incubated for 12 hours at 4°C. Secondary Antibody was a Biotin Goat Anti-Mouse at 1/2000 dilution incubated for 1 hour at room temperature. Counterstain was Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at room temperature. Magnification: 40x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    ICC/IF image of ab53497 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53497, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    All lanes: Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497) at 1 µg/mL

    All lanes: Western blot - Recombinant human Hsp90 beta protein (Active) (Recombinant human Hsp90 beta protein (Active) ab80033) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Exposure time: 8min

  • Flow Cytometry - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Flow Cytometry - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Overlay histogram showing HeLa cells stained with ab53497 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53497, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Paraffin-embedded mouse backskin (epidermis) tissue fixed with Bouin's fixative, stained for Hsp90 beta using ab53497 at 1/100 dilution in immunohistochemical analysis. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a FITC-conjugated goat anti-mouse (green) at 1/50 dilution ncubated for 1 hour at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsp90 beta using ab53497 at 1/10000 in immunohistochemical analysis. Primary antibody was incubated for 12 hours at 4°C. Secondary antibody was an Alexa Fluor® 555 goat anti-mouse (red) at 1/5000 dilution incubated for 1 hour at room temperature.

    40x magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    ab53497 at 100,000 dilution staining Hsp90 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    ab53497 at 100,000 dilution staining Hsp90 beta in mouse colon tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. An Alexa Fluor® 568 conjugated secondary antibody was used at 1/10 dilution.

  • Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    All lanes: Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Lane 1: Hsp90 beta protein at 2 µg

    Lane 2: Hsp90 alpha protein at 2 µg

    Predicted band size: 83 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com