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Mouse Monoclonal HS90B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Chinese hamster, Rat, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSP90AB1.

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Images

Western blot - Anti-Hsp90 beta antibody [K3701] (AB82584), expandable thumbnail
  • Western blot - Anti-Hsp90 beta antibody [K3701] (AB82584), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [K3701] (AB82584), expandable thumbnail

Key facts

Isotype

IgM

Host species

Mouse

Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human HSP90AB1. Database link P08238

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Rat
Tested
Expected
Chinese hamster
Tested
Expected

Tested
Tested

Species

Chinese hamster

Dilution info

1/1000

Notes

1/1000 (colorimetric).

Species

Rat

Dilution info

1/1000

Notes

1/1000 (colorimetric).

Species

Human

Dilution info

1/1000

Notes

1/1000 (colorimetric).

Tested
Tested

Species

Human

Dilution info

1/10

Notes

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Chinese hamster, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:26991466, PubMed:27295069). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. They first alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823). Involved in the translocation into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) of leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).(Microbial infection) Binding to N.meningitidis NadA stimulates monocytes (PubMed:21949862). Seems to interfere with N.meningitidis NadA-mediated invasion of human cells (Probable).

Alternative names

Recommended products

Mouse Monoclonal HS90B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Chinese hamster, Rat, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSP90AB1.

Key facts

Isotype

IgM

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human HSP90AB1. Database link P08238
Clone number

K3701

Purification technique

Precipitation Ammonium Sulphate

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hsp90 beta also known as Hsp90AB1 or Hsp90 protein is a heat shock protein of approximately 90 kDa. It is a molecular chaperone found in most eukaryotic cells. Hsp90 beta helps in the proper folding stabilization and degradation of many proteins. Unlike its isoform Hsp90 alpha Hsp90 beta has a more stable expression and is not typically induced by stress. This protein is primarily localized in the cytosol but can also be present in other cellular compartments depending on the cellular state.

Biological function summary

Hsp90 beta functions to maintain protein homeostasis and cellular integrity. It forms part of a multi-protein chaperone complex which includes cochaperones such as Hop Hsp70 and p23 necessary for its full functionality. Hsp90 beta supports the maturation of steroid hormone receptors kinases and other client proteins. It plays an important role in the cell cycle regulation through its interaction with various proteins ensuring proper cell division and growth.

Pathways

Hsp90 beta is deeply involved in signal transduction and cellular stress response pathways. Its interaction with the Akt pathway is significant for cell survival signals. Hsp90 beta also participates in the MAP kinase pathway affecting cell growth and differentiation. The protein associates with multiple kinases including RAF and Src which are important for downstream signaling.

Associated diseases and disorders

The dysregulation of Hsp90 beta is associated with cancer and neurodegenerative diseases. In cancer Hsp90 beta stabilizes many oncoproteins making it a potential therapeutic target for inhibiting tumor growth. It interacts with client proteins like the proto-oncogene c-Src promoting tumorigenesis. In neurodegenerative disorders such as Alzheimer's disease improper interaction between Hsp90 beta and tau proteins can contribute to disease progression impacting neuronal function.

Product promise

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3 product images

  • Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)

    Lanes 1 - 4: Merged signal (red and green). Green - ab82584 observed at 85 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

    ab82584 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82584 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution

    Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: HSP90AB1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: Saos-2 (Human osteosarcoma cell line) whole cell lysate at 20 µg

    Lane 4: HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 85 kDa

  • Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584), expandable thumbnail

    Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)

    All lanes: Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution

    Lane 1: HeLa cell lysate

    Lane 2: PC12 cell lysate

    Lane 3: CHO-K1 cell lysate

    Lane 4: Hsp90 Native Protein

    Lane 5: Hsp90 alpha Recombinant Protein (negative control)

    Predicted band size: 83 kDa

    Observed band size: 83 kDa

  • Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [K3701] (ab82584), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [K3701] (ab82584)

    ab82584, at 10 μg/ml, staining p90 beta in human colon cancer Caco-2 cells by flow cytometry (right). Isotype control antibody (left).

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Product protocols

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