JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB200838

Anti-HSPA12A antibody [EPR16763]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal HSPA12A antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

KIAA0417, HSPA12A, Heat shock 70 kDa protein 12A, Heat shock protein family A member 12A

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] (AB200838)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U87-MG (human glioblastoma) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on U87-MG cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] (AB200838)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • IP

Supplier Data

Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] (AB200838)

HSPA12A was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab200838 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200838 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal brain whole cell lysate 10 μg (Input). Lane 2 : ab200838 IP in Human fetal brain whole cell lysate. Lane 3 : Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200838 in Human fetal brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] (ab200838)

Predicted band size: 75 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat cerbral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (AB200838)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cerbral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • WB

Supplier Data

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 3min

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • WB

Supplier Data

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 3min

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • WB

Supplier Data

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution

Lane 1:

HEK293 (Human embryonic kidney) cell lysate at 20 µg

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 3min

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)
  • WB

Supplier Data

Western blot - Anti-HSPA12A antibody [EPR16763] (AB200838)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16763

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB236146

Anti-HSPA12A antibody [EPR16763] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

  • 5
  • 6
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPA12A also known as Heat Shock Protein Family A (Hsp70) Member 12A is a member of the Hsp70 family of proteins involved in stress response. This protein has an approximate molecular mass of 80 kDa. HSPA12A shows expression predominantly in endothelial cells where it plays a significant role. Like other Hsp70 proteins it assists in protein folding assembly and transport within cells. HSPA12A's activity supports the maintenance of cellular proteostasis under stress conditions facilitating correct protein conformation and preventing aggregation.
Biological function summary

HSPA12A participates in processes that maintain vascular homeostasis. It may be involved in a complex with other Hsp70 proteins to provide cellular protection against various stressors. The precise composition and roles of these complexes remain under investigation. Its activity as a molecular chaperone helps protect cells from apoptosis and supports the repair of damaged proteins which is important for normal cell function and survival in stressful environments.

Pathways

HSPA12A is involved in key pathways related to cellular stress responses and cytoprotection. It interacts with signaling pathways such as the unfolded protein response and the oxidative stress response. These pathways include interactions with other chaperone proteins like Hsp90 that facilitate cellular adaptation to adverse conditions. Its involvement in these pathways suggests that HSPA12A plays a role in regulating cellular stress mechanisms and promoting recovery after stress exposure.

HSPA12A appears to have a connection to cardiovascular diseases and certain neurodegenerative disorders. In cardiovascular diseases its expression in endothelial cells suggests it may contribute to vascular integrity and function. Furthermore HSPA12A may have connections to neurodegenerative disorders as it assists in protecting neurons from protein aggregation and apoptosis. These links might involve interactions with proteins like alpha-synuclein where its chaperone role could influence the disease progression. Understanding these relationships could provide new insights into therapeutic strategies targeting these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.
See full target information HSPA12A
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:486-496 PubMed39722572

2024

The host gene regulates HBV replication via HBV PRE-induced nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Wang,Jing Li,Wentao Xie,Zhongliang Shen,Jingwen Wu,Richeng Mao,Mengji Lu,Jiming Zhang

Drug development research 85:e22130 PubMed37942840

2023

Heat-shock protein A12A attenuates oxygen-glucose deprivation/reoxygenation-induced human brain microvascular endothelial cell dysfunction via PGC-1α/SIRT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Shouyin Shen,Haiyan Shen

Molecules (Basel, Switzerland) 27: PubMed36014574

2022

MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Xu,Haitong Zhang,Huangting Li,Bo Liu,Rongrong Li,Jinjin Zhang,Xiaodong Song,Changjun Lv,Hongbo Li,Mingwei Chen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com